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TitleDisclosing the complexity involved in phage-biofilm interaction: the case study of a Sep1virus phage infecting S. epidermidis
Author(s)Melo, Luís D. R.
Pinto, Graça
Oliveira, Fernando E.
França, Ângela Maria Oliveira Sousa
Boas, Diana Patrícia Andrade Vilas
Almeida, Carina
Sillankorva, Sanna
Cerca, Nuno
Azeredo, Joana
Issue date6-Jul-2017
CitationMelo, Luís D. R.; Pinto, Graça; Oliveira, Fernando E.; França, Angela; Vilas Boas, Diana; Almeida, Carina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, Joana, Disclosing the complexity involved in phage-biofilm interaction: the case study of a Sep1virus phage infecting S. epidermidis. Book of Abstracts of CEB Annual Meeting 2017. Braga, 6 July, 52, 2017. ISBN: 978-989-97478-8-3
Abstract(s)[Excerpt] Staphylococcus epidermidis is a major causative agent of nosocomial infections, mainly associated with the use of indwelling devices, on which this bacterium forms structures known as biofilms. Due to biofilms high tolerance to antibiotics, virulent bacteriophages have been suggested as novel anti-biofilm therapeutic agents. In this study, we used the S. epidermidis-specific phage phiIBB-SEP1 (SEP1) [1] and evaluated its activity against biofilms. Despite its broad host spectrum and high activity against exponential phase cells, the same was not observed for cells encased in a biofilm structure. To understand the underlying factors impairing SEP1 inefficacy against biofilms, we tested this phage against distinct bacterial populations. Interestingly, SEP1 was able to infect late stationary-phase (dormant), persister and biofilm-released cells, suggesting that the inefficacy for biofilm control resulted from the biofilm structure. To demonstrate this hypothesis, SEP1 activity was tested against clusters of cells from scraped biofilms resulting in a 2 orders-of-magnitude reduction in the number of viable cells, after six hours of infection. Additionally, LIVE/DEAD staining allowed the observation that stationaryphase cells responded to phage addition, as determined by the increase in SYBR medium fluorescence intensity, which can be related with an increase on the cell metabolic activity. [...]
DescriptionBook of Abstracts of CEB Annual Meeting 2017
Publisher version
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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