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TitleExpression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach
Author(s)Bessa, Paulo C.
Pedro, A.
Nobre, Alexandra
Dungl, P.
Banerjee, A.
Dopler, D.
Klosch, B.
Redl, H.
Casal, Margarida
Reis, R. L.
Issue date2006
Abstract(s)Bone tissue engineering has been an increasing field of research during the last years. The ideal approach for a regenerative application would consist in the use of cells from the patient, scaffolding materials and differentiation growth factors. Bone morphogenetic protein-2 (BMP-2) is one such growth factors with a strong ability to induce new bone and cartilage formation and has been used as a powerful osteoinductive component of several late-stage tissue engineering products for bone grafting. In this work, we aimed at obtaining high yields of human recombinant BMP-2 in a stable, pure and biologically active form by use of a new bacteria expression system that circumvents the disadvantages of conventional recombinant protein preparation methods and to perform a study of the stability conditions and functionality of these peptides in vitro in human mesenchymal stem cells and C2C12 murine cell line.
AccessOpen access
Appears in Collections:DBio - Comunicações/Communications in Congresses

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