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dc.contributor.authorFerreira, Daniellypor
dc.contributor.authorNobre, Alexandrapor
dc.contributor.authorSilva, Marta Luisapor
dc.contributor.authorFaria-Oliveira, Fábiopor
dc.contributor.authorTulha, Joanapor
dc.contributor.authorFerreira, Céliapor
dc.contributor.authorLucas, Cândidapor
dc.description.abstractThe closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding this permease, AY347871.2. This was retrieved from D. fabryi gDNA using a degenerate primer PCR strategy, based on conserved regions from the amino acid sequences of three well-characterized bacterial xylose/H(+) symporters. This sequence is 86% identical to another, DEHA2C11374p from D. hansenii type strain. DEHA2C11374p was conceptually ascribed to the major facilitator superfamily. The putative amino acid sequence of AY347871.2 and DEHA2C11374p presented a hydrophobicity pattern compatible with plasma membrane proteins. The last was functionally expressed in Saccharomyces cerevisiae. The sensitivity of transport activity to a protonophore confirmed its dependence on proton motive force, as expected from a symporter. We named D. fabryi AY347871.2 and D. hansenii DEHA2C11374p as XYLH from Xylose/H(+) symport. Based on the very high similarity, we suggested that Scheffersomyces stipitis Xut3 and Aspergillus nidulans AN8400.2 may also encode xylose high-affinity permeases.por
dc.description.sponsorshipD.F. was a Master Student supported by Alβan Office Grant E06M103958BR; C.F. was a Post-Doc FCT SFRH/BPD720908/2004/W7D7. We thank F. Gírio from the LNEG, Lisbon, Portugal, for kindly supplying CCMI 941 industrial yeast strain. The work was supported by internal budgets from CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, which is funded by FEDER, through COMPETE – Programa Operacional Factores de Competitividade, and national funds, through FCT – Fundação para a Ciência e a Tecnologia - PEst-C/BIA/UI4050/2011.por
dc.publisherJohn Wiley & Sonspor
dc.subjectProton symporterpor
dc.subjectHigh-affinity permeasepor
dc.titleXYLH encodes a xylose/H+ symporter from the highly related yeast species Debaryomyces fabryi and Debaryomyces hanseniipor
dc.subject.fosCiências Naturais::Ciências Biológicaspor
dc.subject.wosScience & Technologypor
sdum.journalFEMS Yeast Researchpor
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