Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/53776

TitleComparative analysis between biofilm formation and gene expression in Staphylococcus epidermidis isolates
Author(s)Freitas, Ana Isabel
Lopes, N.
Oliveira, Fernando E.
Brás, Susana
França, Angela
Vasconcelos, C.
Vilanova, Manuel
Cerca, Nuno
Keywordsbiofilm development
commensal and clinical S. epidermidis
gene expression quantification
matrix composition and biofilm organization
Issue dateMar-2018
PublisherFuture Medicine Ltd
JournalFuture Microbiology
CitationFreitas, Ana Isabel; Lopes, N.; Oliveira, Fernando E.; Brás, Susana; França, Angela; Vasconcelos, C.; Vilanova, Manuel; Cerca, Nuno, Comparative analysis between biofilm formation and gene expression in Staphylococcus epidermidis isolates. Future Microbiology, 13(4), 415-427, 2018
Abstract(s)Aim: To understand the relationship between ica, aap and bhp gene expression and the implications in biofilm formation in selected clinical and commensal Staphylococcus epidermidis isolates. Material & methods: Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, biofilm spatial organization and expression of biofilm-related genes. Results: On polysaccharide intercellular adhesin-dependent biofilms, aap and bhp contributions for the biofilm growth were negligible, despite very high levels of expression. In contrast, smaller increases in icaA expression contributed significantly to biofilm growth. Interestingly, no biological differences were observed between clinical and commensal strains. Conclusion: These results reinforce the concept that S. epidermidis is an 'accidental pathogen,' and that the ica operon is the main mechanism of biofilm formation in clinical and commensal isolates. © 2018 Future Medicine Ltd.
TypeArticle
URIhttp://hdl.handle.net/1822/53776
DOI10.2217/fmb-2017-0140
ISSN1746-0913
e-ISSN1746-0921
Publisher versionhttp://www.futuremedicine.com/loi/fmb
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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