Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/53019

TitleHarnessing the power of PCR molecular fingerprinting methods and next generation sequencing for understanding structure and function in microbial communities
Author(s)Phadke, Sujal
Salvador, Andreia F.
Alves, J. I.
Bretschger, Orianna
Alves, M. M.
Pereira, M. A.
KeywordsPolymerase chain reaction (PCR)
Microbiome Canonical correspondence analysis (CCA)Restriction fragment length polymorphism (T-RFLP)
Denaturing gradient gel electrophoresis (DGGE)
Next generation sequencing (NGS)
Canonical correspondence analysis (CCA)
Microbiome
Restriction fragment length polymorphism (T-RFLP)
Issue date2017
PublisherSpringer
JournalMethods in Molecular Biology
CitationPhadke, Sujal; Salvador, Andreia F.; Alves, J. I.; Bretschger, Orianna; Alves, M. Madalena; Pereira, M. Alcina, Harnessing the power of PCR molecular fingerprinting methods and next generation sequencing for understanding structure and function in microbial communities. In Lucília Domingues (Ed.), PCR: Methods and Protocols, Vol. Methods in Molecular Biology 1620, New York: Springer, 2017. ISBN: 978-1-4939-7060-5, 225-248
Abstract(s)Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques.
TypeBook part
URIhttp://hdl.handle.net/1822/53019
ISBN978-1-4939-7060-5
DOI10.1007/978-1-4939-7060-5_16
ISSN1064-3745
Publisher versionhttps://link.springer.com/protocol/10.1007/978-1-4939-7060-5_16
Peer-Reviewedyes
AccessRestricted access (Author)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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