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https://hdl.handle.net/1822/52886
Título: | Interactions between Candida albicans and Candida glabrata in biofilms: influence of the strain type, culture medium and glucose supplementation |
Autor(es): | Hosida, Thayse Yumi Cavazana, Thamires Priscila Henriques, Mariana Pessan, Juliano Pelim Delbem, Alberto Carlos Botazzo Monteiro, D. |
Palavras-chave: | Biofilms Candida albicans Candida glabrata Microbial interactions |
Data: | Abr-2018 |
Editora: | Wiley-Blackwell |
Revista: | Mycoses |
Citação: | Hosida, Thayse Yumi; Cavazana, Thamires Priscila; Henriques, Mariana; Pessan, Juliano Pelim; Delbem, Alberto Carlos Botazzo; Monteiro, D., Interactions between Candida albicans and Candida glabrata in biofilms: influence of the strain type, culture medium and glucose supplementation. Mycoses, 61(4), 270-278, 2018 |
Resumo(s): | The relationship among Candida species may be influenced by several factors. Thus, this study evaluated the interactions between Candida albicans and Candida glabrata in biofilms, varying the strain type, culture medium and glucose supplementation. Biofilms were formed for 48 h in SDB or RPMI 1640, supplemented with 0, 1 or 5% glucose. Each strain of C. albicans was combined with two strains of C. glabrata, generating 4 biofilm associations, which were quantified by colony-forming units (CFUs), total biomass and metabolic activity. Data were analyzed by ANOVA and Tukey's HSD test ( = 0.05). For CFUs, all associations were classified as indifferent for biofilms formed in RPMI 1640, while for SDB the interactions were antagonistic for C. albicans and indifferent for C. glabrata. The association of reference strains resulted in a dual-species biofilm with biomass significantly higher than that observed for each single biofilm developed in SDB. The metabolic activity of dual-species biofilms did not significantly differ from that found for single ones, except for co-culture of the reference strains. Glucose supplementation and culture media had a significant influence on all parameters. In conclusion, the type strain, culture medium and glucose supplementation influenced the interactions between C. albicans and C. glabrata. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/52886 |
DOI: | 10.1111/myc.12738 |
ISSN: | 0933-7407 |
e-ISSN: | 1439-0507 |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0507 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47363_1.pdf | 1,27 MB | Adobe PDF | Ver/Abrir |