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TitleImmobilization of bacteriophage in wound-dressing nanostructure
Author(s)Nogueira, F.
Karumidze, Natia
Kusradze, Ia
Goderdzishvili, Marina
Teixeira, Pilar
Gouveia, Isabel C.
Issue dateNov-2017
JournalNanomedicine: Nanotechnology, Biology, and Medicine
CitationNogueira, F.; Karumidze, Natia; Kusradze, Ia; Goderdzishvili, Marina; Teixeira, Pilar; Gouveia, Isabel C., Immobilization of bacteriophage in wound-dressing nanostructure. Nanomedicine-Nanotechnology Biology and Medicine, 13(8), 2475-2484, 2017
Abstract(s)Opportunistic bacteria that cause life-threatening infections are still a central problem associated with a healthcare setting. Bacteriophage capsid immobilization on nanostructured polymers maximizes its tail exposure and looks promising in applications toward skin-infections as alternative to antibiotics standardly used. The main goal of this work was to investigate the covalent immobilization of vB_Pae_Kakheti25 bacteriophage capsid on polycaprolactone (PCL) nanofibers (non-woven textile), as a potential effective antimicrobial, laundry resistant and non-toxic dressing for biomedical use. Surface analyses showed that the immobilization of vB_Pae_Kakheti25 bacteriophage capsid on PCL nanofibres oriented bacteriophage tails to interact with bacteria. Furthermore, antimicrobial assays showed a very effective 6 log bacterial reduction, which was equivalent to 99.9999%, after immediate and 2 hours of contact, even following 25 washing cycles (due to covalent bond). The activity of PCL-vB_Pae_Kakheti25 against P. aeruginosa was immediate and its reduction was complete.
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AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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