Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/52720

TitleImmobilization of bacteriophage in wound-dressing nanostructure
Author(s)Nogueira, F.
Karumidze, Natia
Kusradze, Ia
Goderdzishvili, Marina
Teixeira, Pilar
Gouveia, Isabel C.
Issue dateNov-2017
PublisherElsevier
JournalNanomedicine: Nanotechnology, Biology, and Medicine
CitationNogueira, F.; Karumidze, Natia; Kusradze, Ia; Goderdzishvili, Marina; Teixeira, Pilar; Gouveia, Isabel C., Immobilization of bacteriophage in wound-dressing nanostructure. Nanomedicine-Nanotechnology Biology and Medicine, 13(8), 2475-2484, 2017
Abstract(s)Opportunistic bacteria that cause life-threatening infections are still a central problem associated with a healthcare setting. Bacteriophage capsid immobilization on nanostructured polymers maximizes its tail exposure and looks promising in applications toward skin-infections as alternative to antibiotics standardly used. The main goal of this work was to investigate the covalent immobilization of vB_Pae_Kakheti25 bacteriophage capsid on polycaprolactone (PCL) nanofibers (non-woven textile), as a potential effective antimicrobial, laundry resistant and non-toxic dressing for biomedical use. Surface analyses showed that the immobilization of vB_Pae_Kakheti25 bacteriophage capsid on PCL nanofibres oriented bacteriophage tails to interact with bacteria. Furthermore, antimicrobial assays showed a very effective 6 log bacterial reduction, which was equivalent to 99.9999%, after immediate and 2 hours of contact, even following 25 washing cycles (due to covalent bond). The activity of PCL-vB_Pae_Kakheti25 against P. aeruginosa was immediate and its reduction was complete.
TypeArticle
URIhttp://hdl.handle.net/1822/52720
DOI10.1016/j.nano.2017.08.008
ISSN15499634
e-ISSN1549-9642
Publisher versionhttp://www.nanomedjournal.com/
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_46948_1.pdf
  Restricted access
2,07 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID