Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/51655

TitleSUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana
Author(s)Castro, Pedro Humberto
Couto, Daniel
Freitas, Sara
Verde, Nuno
Macho, Alberto P.
Huguet, Stéphanie
Botella, Miguel Angel
Ruiz-Albert, Javier
Tavares, R. M.
Bejarano, Eduardo Rodríguez
Azevedo, Herlânder Anselmo Queirós Pereira
KeywordsAbscisic Acid
Arabidopsis
Arabidopsis Proteins
Gene Expression Regulation, Plant
Germination
Osmoregulation
ABA
Osmotic stress
Plant development
Stomatal aperture
SIZ1
SUMO proteases
Issue dateSep-2016
PublisherSpringer
JournalPlant Molecular Biology
Abstract(s)Sumoylation is an essential post-translational regulator of plant development and the response to environmental stimuli. SUMO conjugation occurs via an E1-E2-E3 cascade, and can be removed by SUMO proteases (ULPs). ULPs are numerous and likely to function as sources of specificity within the pathway, yet most ULPs remain functionally unresolved. In this report we used loss-of-function reverse genetics and transcriptomics to functionally characterize Arabidopsis thaliana ULP1c and ULP1d SUMO proteases. GUS reporter assays implicated ULP1c/d in various developmental stages, and subsequent defects in growth and germination were uncovered using loss-of-function mutants. Microarray analysis evidenced not only a deregulation of genes involved in development, but also in genes controlled by various drought-associated transcriptional regulators. We demonstrated that ulp1c ulp1d displayed diminished in vitro root growth under low water potential and higher stomatal aperture, yet leaf transpirational water loss and whole drought tolerance were not significantly altered. Generation of a triple siz1 ulp1c ulp1d mutant suggests that ULP1c/d and the SUMO E3 ligase SIZ1 may display separate functions in development yet operate epistatically in response to water deficit. We provide experimental evidence that Arabidopsis ULP1c and ULP1d proteases act redundantly as positive regulators of growth, and operate mainly as isopeptidases downstream of SIZ1 in the control of water deficit responses.
TypeArticle
URIhttp://hdl.handle.net/1822/51655
DOI10.1007/s11103-016-0500-9
ISSN0167-4412
Peer-Reviewedyes
AccessRestricted access (Author)
Appears in Collections:DBio - Artigos/Papers

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