Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/51511

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dc.contributor.authorOliveira, Hugopor
dc.contributor.authorCosta, Ana Ritapor
dc.contributor.authorKonstantinidis, Nicopor
dc.contributor.authorFerreira, A.por
dc.contributor.authorAkturk, Ergunpor
dc.contributor.authorSillankorva, Sannapor
dc.contributor.authorNemec, Alexanderpor
dc.contributor.authorShneider, Mikhailpor
dc.contributor.authorDötsch, Andreaspor
dc.contributor.authorAzeredo, Joanapor
dc.date.accessioned2018-03-05T11:58:10Z-
dc.date.available2018-03-05T11:58:10Z-
dc.date.issued2017-12-
dc.identifier.citationOliveira, Hugo; Costa, Ana Rita; Konstantinidis, Nico; Ferreira, A.; Akturk, Ergun; Sillankorva, Sanna; Nemec, Alexander; Shneider, Mikhail; Dötsch, Andreas; Azeredo, Joana, Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains. Environmental Microbiology, 19(12), 5060-5077, 2017por
dc.identifier.issn1462-2912por
dc.identifier.urihttp://hdl.handle.net/1822/51511-
dc.description.abstractBacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm). Notably, the alignment of the newly sequenced phage genomes (40-41 kb of DNA length) and all Acinetobacter podoviruses deposited in Genbank has shown high synteny, regardless of the date and source of isolation that spans from America to Europe and Asia. Interestingly, the C-terminal pectate lyase domain of these phage tail fibers is often the only difference found among these viral genomes, demonstrating a very specific genomic variation during the course of their evolution. We proved that the pectate lyase domain is responsible for phage depolymerase activity and binding to specific Acinetobacter bacterial capsules. We discuss how this mechanism of phage-host co-evolution impacts the tail specificity apparatus of Acinetobacter podoviruses.por
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI01–0145-FEDER-006684) and the Project PTDC/BBB-BSS/6471/2014 (POCI-01–0145-FEDER-016678). This work was also supported by BioTecNorte operation (NORTE-01–0145FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. We acknowledge Dr. Lenie Dijkshoorn (Leiden Medical Center) for the provision of some strains (LUH or RUH designations). AFM was performed at i3s- Instituto de Investigação e Inovação para a Sa ude at the Biointerfaces and Nanotechnology platform. SS is an FCT Investigator (IF/01413/ 2013). HO and ARC acknowledge FCT for grants SFRH/BPD/ 111653/2015 and SFRH/BPD/94648/2013 respectively. The authors declare that they have no competing financial interests.por
dc.language.isoengpor
dc.publisherWiley-Blackwellpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147337/PTpor
dc.relationPTDC/BBB-BSS/6471/2014por
dc.relationSFRH/BPD/111653/2015por
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94648%2F2013/PTpor
dc.rightsopenAccesspor
dc.subjectAcinetobacter calcoaceticus-Acinetobacter baumannii complexpor
dc.subjectbacteriophagepor
dc.subjectcomparative genomicspor
dc.subjectdepolymerasepor
dc.subjectcapsulepor
dc.titleAbility of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domainspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920por
dc.commentsCEB47057por
oaire.citationStartPage5060por
oaire.citationEndPage5077por
oaire.citationIssue12por
oaire.citationConferencePlaceUnited Kingdom-
oaire.citationVolume19por
dc.date.updated2018-01-03T23:42:01Z-
dc.identifier.essn1462-2920por
dc.identifier.doi10.1111/1462-2920.13970por
dc.identifier.pmid29076652por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technologypor
sdum.journalEnvironmental Microbiologypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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