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TitleKinetic model evaluation of ethanol fermentation from lactose
Author(s)Juraščík, Martin
Klein, Jaroslav
Teixeira, J. A.
Markoš, J.
KeywordsEthanol fermentation of lactose
Recombinant Saccharomyces cerevisiae
Kinetic model
Kinetic parameters estimation
Issue date2006
CitationMARKOS, J. ; STEFUCA, V. , ed. lit. – “Proceedings of the International Conference of Slovak Society of Chemical Engineering, 33, Tatranské Matliare, Slovakia, 2006”. [S.l. : s.n.], 2006. ISBN 80-227-2409-2. p. 037-1.
Abstract(s)This work presents a multi-route, nonstructural kinetic model for characterization of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol) and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g dm ˉ³to 50 g dmˉ³. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass and dissolved oxygen concentrations) was achieved.
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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