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TitleNature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration
Author(s)Kook, Yeon Ji
Tian, Jingwen
Jeon, Yoo Shin
Choi, Min Jung
Song, Jeong Eun
Park, Chan Hum
Reis, R. L.
Khang, Gilson
KeywordsBone marrow stromal cells (BMSCs)
Bone regeneration
Ducks feet collagen (DC)
Epigallocatechin gallate (EGCG)
Hydroxyapatite (HAp)
Issue date2018
PublisherTaylor & Francis
JournalJournal of Biomaterials Science. Polymer Edition
CitationKook Y. J., Tian J., Jeon Y. S., Choi M. J., Song J. E., Park C. H., Reis R. L., Khang G. Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration, Journal Of Biomaterials Science-polymer Edition, Vol. -, Issue -, pp. 1-13, doi:10.1080/09205063.2017.1414480, 2018
Abstract(s)Scaffolds mimicking structural and chemical characteristics of the native bone tissues are critical for bone tissue engineering. Herein, we have developed and characterized epigallocatechin gallate/duck's feet collagen/hydroxyapatite (EGCG/DC/HAp) composite sponges that enhanced the bone tissue regeneration. The three-dimensional composite sponges were synthesized by loading various amounts (i.e. 1, 5 and 10 μM) of EGCG to duck feet derived collagen followed by freeze-drying and then coating with hydroxyapatite. Several measuremental techniques were employed to examine the properties of the as-fabricated composite sponges including morphology and structure, porosity, compressive strength, etc. and as well compared with pristine duck feet derived collagen. SEM observations of EGCG/DC/HAp sponges showed the formation of a highly porous collagen matrix with EGCG embodiment. The porosity and pore size of sponges were found to increase by high EGCG content. The compressive strength was calculated as 3.54 ± 0.04, 3.63 ± 0.03, 3.89 ± 0.05, 4.047 ± 0.05 MPa for 1, 5 and 10 μM EGCG/DC/HAp sponges, respectively. Osteoblast-like cell (BMSCs isolated from rabbit) culture and in vivo experiments with EGCG/DC/HAp sponges implanted in nude mouse followed by histological staining showed enhanced cell internalization and attachment, cell proliferation, alkaline phosphatase expressions, indicating that EGCG/DC/HAp sponges have ahigh biocompatibility. Moreover, highEGCG content in the EGCG/DC/HAp sponges have led to increased cellular behavior. Collectively, the 5 μM of EGCG/DC/HAp sponges were suggested as the potential candidates for bone tissue regeneration.
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AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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