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https://hdl.handle.net/1822/51339
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Campo DC | Valor | Idioma |
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dc.contributor.author | Chowdury, Helena H. | por |
dc.contributor.author | Cerqueira, Susana Isabel Ribeiro | por |
dc.contributor.author | Sousa, Nuno | por |
dc.contributor.author | Oliveira, J. M. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Zorec, Robert | por |
dc.date.accessioned | 2018-03-01T14:50:36Z | - |
dc.date.available | 2018-03-01T14:50:36Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | Chowdury H. H., Cerqueira S. R., Sousa N., Oliveira J. M., Reis R. L., Zorec R. The Uptake, Retention and Clearance of Drug-Loaded Dendrimer Nanoparticles in Astrocytes - Electrophysiological Quantification, Biomaterials Science, Vol. 6, Issue 2, pp. 388-397, doi:10.1039/C7BM00886D, 2018 | por |
dc.identifier.issn | 2047-4830 | por |
dc.identifier.uri | https://hdl.handle.net/1822/51339 | - |
dc.description.abstract | Nanoparticle-based drug delivery systems may impose risks to patients due to potential toxicity associated with lack of clearance from cells or prolonged carrier-cell retention. This work evaluates vesicular cell uptake, retention and the possible transfer of endocytosed methylprednisolone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles (NPs) into secretory vesicles of rat cultured astrocytes. Cells were incubated with NPs and unitary vesicle fusions/fissions with the plasma membrane were monitored employing high resolution membrane capacitance measurements. In NPs-treated cells the frequency of unitary exocytotic events was significantly increased. The presence of NPs also induce an increase in the size of exocytotic vesicles interacting with the plasma membrane, which exhibit transient fusion with prolonged fusion pore dwell-time. Live-cell confocal imaging revealed that once NPs internalize into endocytotic compartments they remain in the cell for 7 days, although a significant proportion of these merge with secretory vesicles destined for exocytosis. Co-localization studies show the route of clearance of NPs from cells via the exocytotic pathway. These findings bring new insight into the understanding of the intracellular trafficking and biological interactions of drug-loaded dendrimer NPs targeting astrocytes. | por |
dc.description.sponsorship | The study was supported by the Slovenian Research Agency (grants P3 310, J3 6790, J3 7605) and the European Science Foundation COST STSM grant attributed to S.R. Cerqueira. The authors would also like to acknowledge the funds from the Portuguese Foundation for Science and Technology (fellowships to S.R.C. SFRH/BD/48406/2008) and the European Union's Seventh Framework Programme (FP7/2007-2013, grant agreement no. REGPOT-CT2012-316331-POLARIS). J.M.O. also thanks the FCT for the funds provided under the program Investigador FCT (IF/00423/2012 and IF/01285/2015). | - |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | - |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F48406%2F2008/PT | - |
dc.rights | openAccess | por |
dc.subject | Dendrimer nanoparticles | por |
dc.subject | Exocytosis/endocytosis | por |
dc.subject | Astrocytes | por |
dc.subject | High-resolution membrane capacitance | por |
dc.subject | Live confocal imaging | por |
dc.title | The uptake, retention and clearance of drug-loaded dendrimer nanoparticles in astrocytes - electrophysiological quantification | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://dx.doi.org/10.1039/C7BM00886D | por |
dc.comments | http://3bs.uminho.pt/node/19294 | por |
oaire.citationStartPage | 388 | por |
oaire.citationEndPage | 397 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2018-02-09T17:20:07Z | - |
dc.identifier.eissn | 2047-4849 | por |
dc.identifier.doi | 10.1039/C7BM00886D | por |
dc.identifier.pmid | 29336451 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomaterials Science | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19294-c7bm00886d.pdf | 592,73 kB | Adobe PDF | Ver/Abrir |