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TitleFab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
Author(s)Ohradanova-Repic, Anna
Nogueira, E.
Hartl, Ingrid
Gomes, Andreia C.
Preto, Ana
Steinhuber, Eva
Mühlgrabner, Vanessa
Repic, Marko
Kuttke, Mario
Zwirzitz, Alexander
Prouza, Marek
Suchanek, Miloslav
Wozniak-Knopp, Gordana
Horejsi, Vaclav
Schabbauer, Gernot
Cavaco-Paulo, Artur
Stockinger, Hannes
Keywordsactive targeting
liposome functionalization
antibody engineering
recombinant Fab antibody fragment
Issue date2018
JournalNanomedicine: Nanotechnology, Biology, and Medicine
CitationOhradanova-Repic, Anna; Nogueira, E.; Hartl, Ingrid; Gomes, Andreia C.; Preto, Ana; Steinhuber, Eva; Mühlgrabner, Vanessa; Repic, Marko; Kuttke, Mario; Zwirzitz, Alexander; Prouza, Marek; Suchanek, Miloslav; Wozniak-Knopp, Gordana; Horejsi, Vaclav; Schabbauer, Gernot; Cavaco-Paulo, Artur; Stockinger, Hannes, Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells. Nanomedicine-Nanotechnology Biology and Medicine, 14(1), 123-130, 2018. doi: 10.1016/j.nano.2017.09.003
Abstract(s)Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DBio - Artigos/Papers

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