Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/51155

TitleThe Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae
Author(s)Soares-Silva, Isabel
Ribas, David
Foskolou, Iosifina P.
Barata, Beatriz
Bessa, Daniela
Paiva, Sandra
Queiros, Odilia
Casal, Margarida
KeywordsDebaryomyces hansenii
Yeast
Jen1 lactate transporter family
carboxylic acids transport
Issue date2015
PublisherOxford University Press
JournalFEMS Yeast Research
Abstract(s)We have functionally characterized the four Saccharomyces cerevisiae (Sc) Jen1 homologues of Debaryomyces hansenii (Dh) by heterologous expression in S. cerevisiae. Debaryomyces hansenii cells display mediated transport for the uptake of lactate, acetate, succinate and malate. DHJEN genes expression was detected by RT-PCR in all carbon sources assayed, namely lactate, succinate, citrate, glycerol and glucose. The heterologous expression in the S. cerevisiae W303-1A jen1 Lambda ady2 Lambda strain demonstrated that the D. hansenii JEN genes encode four carboxylate transporters. DH27 gene encodes an acetate transporter (K-m 0.94 +/- 0.17 mM; V-max 0.43 +/- 0.03 nmol s(-1) mg(-1)), DH17 encodes a malate transporter (K-m 0.27 +/- 0.04 mM; V-max 0.11 +/- 0.01 nmol s(-1) mg(-1)) and both DH18 and DH24 encode succinate transporters with the following kinetic parameters, respectively, K-m 0.31 +/- 0.06 mM; V-max 0.83 +/- 0.04 nmol s(-1) mg(-1)and K-m 0.16 +/- 0.02 mM; V-max 0.19 +/- 0.02 nmol s(-1) mg(-1). Surprisingly, no lactate transporter was found, although D. hansenii presents a mediated transport for this acid. This work advanced the current knowledge on yeast carboxylate transporters by characterizing four new plasma membrane transporters in D. hansenii.
TypeArticle
URIhttp://hdl.handle.net/1822/51155
DOI10.1093/femsyr/fov094
ISSN1567-1356
Peer-Reviewedyes
AccessRestricted access (Author)
Appears in Collections:DBio - Artigos/Papers

Files in This Item:
File Description SizeFormat 
Soares Silva 2015.pdf
  Restricted access
3,19 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID