Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/51151

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Campo DCValorIdioma
dc.contributor.authorConde, Arturpor
dc.contributor.authorRegalado, Anapor
dc.contributor.authorRodrigues, Dianapor
dc.contributor.authorMiguel Costa, J.por
dc.contributor.authorBlumwald, Eduardopor
dc.contributor.authorManuela Chaves, M.por
dc.contributor.authorGerós, H.por
dc.date.accessioned2018-02-27T20:51:48Z-
dc.date.issued2015-
dc.identifier.issn0022-0957por
dc.identifier.urihttps://hdl.handle.net/1822/51151-
dc.description.abstractPolyols are important metabolites that often function as carbon and energy sources and/or osmoprotective solutes in some plants. In grapevine, and in the grape berry in particular, the molecular aspects of polyol transport and metabolism and their physiological relevance are virtually unknown to date. Here, the biochemical function of a grapevine fruit mesocarp polyol transporter (VvPLT1) was characterized after its heterologous expression in yeast. This H+-dependent plasma membrane carrier transports mannitol (K-m=5.4 mM) and sorbitol (K-m=9.5 mM) over a broad range of polyols and monosaccharides. Water-deficit stress triggered an increase in the expression of VvPLT1 at the fully mature stage, allowing increased polyol uptake into pulp cells. Plant polyol dehydrogenases are oxireductases that reversibly oxidize polyols into monosaccharides. Mannitol catabolism in grape cells (K-m=30.1 mM mannitol) and mature berry mesocarps (K-m=79 mM) was, like sorbitol dehydrogenase activity, strongly inhibited (50-75%) by water-deficit stress. Simultaneously, fructose reduction into polyols via mannitol and sorbitol dehydrogenases was stimulated, contributing to their higher intracellular concentrations in water-deficit stress. Accordingly, the concentrations of mannitol, sorbitol, galactinol, myo-inositol, and dulcitol were significantly higher in berry mesocarps from water-deficit-stressed Tempranillo grapevines. Metabolomic profiling of the berry pulp by GC-TOF-MS also revealed many other changes in its composition induced by water deficit. The impact of polyols on grape berry composition and plant response to water deficit stress, via modifications in polyol transport and metabolism, was analysed by integrating metabolomics with transcriptional analysis and biochemical approaches.por
dc.description.sponsorshipThis work is supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme-INNOVINE-ref. 311775, and Enoexcel-Norte-07-0124-FEDER-000032), by Portuguese national funds (FCT-Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022692, the research projects PTDC/AGR-AAM/099154/2008 and PTDC/AGR-ALI/100636/2008, and the Will W. Lester Endowment of the University of California. AC was supported by an FCT PhD grant no. SFRH/BD/47699/2008. This work also benefited from the networking activities within the European-funded COST ACTION FA1106 'QualityFruit'. We thank Carlos Conde from the Instituto de Biologia Molecular e Celular (IBMC) for useful help and advice on confocal microscopy and imaging. We also thank Remi Lemoine from the Laboratoire de Physiologie et Biochimie Vegetales, Centre National de la Recherche Scientifique UMR for kindly providing the MaDH4 yeast strain used in the heterologous expression studies.por
dc.language.isoengpor
dc.publisherOxford University Presspor
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/99154/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/100636/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F47699%2F2008/PTpor
dc.rightsclosedAccesspor
dc.subjectGrape berry metabolomepor
dc.subjectosmoprotectionpor
dc.subjectpolyol metabolismpor
dc.subjectpolyol/sugar ratiopor
dc.subjectpolyol transportpor
dc.subjectVvPLT1por
dc.subjectwater-deficit stresspor
dc.titlePolyols in grape berry: transport and metabolic adjustments as a physiological strategy for water-deficit stress tolerance in grapevinepor
dc.typearticle-
dc.peerreviewedyespor
oaire.citationStartPage889por
oaire.citationEndPage906por
oaire.citationIssue3por
oaire.citationVolume66por
dc.date.updated2018-02-27T16:31:55Z-
dc.identifier.doi10.1093/jxb/eru446por
dc.identifier.pmid25433029-
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technology-
sdum.export.identifier3022-
sdum.journalJournal of Experimental Botanypor
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