Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/51056

TitleIntrastrain genomic and phenotypic variability of the commercial Saccharomyces cerevisiae strain Zymaflore VL1 reveals microevolutionary adaptation to vineyard environments
Author(s)Franco-Duarte, Ricardo
Bigey, Frederic
Carreto, Laura
Mendes, Inês Isabel Moreira Moutinho Vieira
Dequin, Sylvie
Santos, Manuel A. S.
Pais, Célia
Schuller, Dorit Elisabeth
KeywordsAdaptation
Saccharomyces cerevisiae
Next-generation sequencing
Micro-evolutionary changes
Phenotypic characterization
Issue date1-Sep-2015
PublisherOxford University Press
JournalFEMS YEAST RESEARCH
CitationFranco-Duarte, R., Bigey, F., Carreto, L., Mendes, I., Dequin, S., Santos, M. A., ... & Schuller, D. (2015). Intrastrain genomic and phenotypic variability of the commercial Saccharomyces cerevisiae strain Zymaflore VL1 reveals microevolutionary adaptation to vineyard environments. FEMS yeast research, 15(6)
Abstract(s)The maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome variations among four isolates of the commercial winemaking strain Saccharomyces cerevisiae Zymaflore VL1 that were re-isolated from vineyards surrounding wineries where this strain was applied during several years, in comparison with the commercial reference strain. Comparative genome hybridization showed amplification of 14 genes among the recovered isolates being related with mitosis, meiosis, lysine biosynthesis, galactose and asparagine catabolism, besides 9 Ty elements. The occurrence of microevolutionary changes was supported by DNA sequencing that revealed 339-427 SNPs and 12-62 indels. Phenotypic screening and metabolic profiles also distinguished the recovered isolates from the reference strain. We herein show that the transition from nutrient-rich musts to nutritionally scarce natural environments induces adaptive responses and microevolutionary changes promoted by Ty elements and by nucleotide polymorphisms that were not detected in the reference strain.
TypeArticle
URIhttp://hdl.handle.net/1822/51056
DOI10.1093/femsyr/fov063
ISSN1567-1356
e-ISSN1567-1364
Publisher versionhttps://academic.oup.com/femsyr/article/15/6/fov063/636190
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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