Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/51034

TitleRegulation of Bax/mitochondria interaction by AKT
Author(s)Simonyan, Lilit
Renault, Thibaud T.
Novais, Maria João da Costa
Sousa, Maria João
Côrte-Real, Manuela
Camougrand, Nadine
Gonzalez, Cécile
Manon, Stéphen
KeywordsAKT
Apoptosis
Bcl-2 family
Mitochondria
Yeast model
Issue date1-Jan-2016
PublisherWiley
JournalFEBS Letters
CitationSimonyan, L., Renault, T. T., Costa Novais, M. J., Sousa, M. J., Côrte‐Real, M., Camougrand, N., ... & Manon, S. (2016). Regulation of Bax/mitochondria interaction by AKT. FEBS letters, 590(1), 13-21
Abstract(s)Bax-dependent mitochondrial permeabilization during apoptosis is controlled by multiple factors, including the phosphorylation by the protein kinase AKT. We used the heterologous co-expression of human Bax and AKT1 in yeast to investigate how the kinase modulates the different steps underlying Bax activation. We found that AKT activated Bax and increased its cellular content. Both effects were dependent on Ser184, but a phosphorylation of this residue did not fully explain the effects of AKT. Additional experiments with mutants substituted on Ser184 suggested that the regulation of Bax dynamic equilibrium between the cytosol and mitochondria might be more tightly regulated by Bcl-xL when Bax is phosphorylated.
TypeArticle
URIhttp://hdl.handle.net/1822/51034
DOI10.1002/1873-3468.12030
ISSN0014-5793
e-ISSN1873-3468
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12030/full
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:DBio - Artigos/Papers

Files in This Item:
File Description SizeFormat 
Simonyan_et_al.pdf
  Restricted access
740,74 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID