Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/50943| Título: | A new method for yeast phagocytosis analysis by flow cytometry |
| Autor(es): | Carneiro, Maria Catarina Santos Vaz, Catarina Oliveira Pais, Célia Sampaio, Paula Pereira, Joana Isabel Carvalho |
| Palavras-chave: | Candida spp Flow cytometry Sytox green Propidium iodide Phagocytosis PAMPs |
| Data: | 2014 |
| Editora: | Elsevier |
| Revista: | Journal of Microbiological Methods |
| Resumo(s): | Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/50943 |
| DOI: | 10.1016/j.mimet.2014.03.013 |
| ISSN: | 0167-7012 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| (2014) J Microbiol Methods .pdf | 1,48 MB | Adobe PDF | Ver/Abrir |
