Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/50848

TitleTaxa-area relationship of aquatic fungi on deciduous leaves
Author(s)Duarte, Sofia Alexandra Ferreira
Cássio, Fernanda
Pascoal, Cláudia
Bärlocher, Felix
KeywordsAquatic organisms
Biodiversity
Fungi
High-Throughput Nucleotide Sequencing
Plant leaves
Issue date2017
PublisherPublic Library of Science
JournalPLoS ONE
CitationDuarte S, Cássio F, Pascoal C, Bärlocher F. 2017. Taxa-area relationship of aquatic fungi on deciduous leaves. PLoS ONE 12(7): e0181545
Abstract(s)One of the fundamental patterns in macroecology is the increase in the number of observed taxa with size of sampled area. For microbes, the shape of this relationship remains less clear. The current study assessed the diversity of aquatic fungi, by the traditional approach based on conidial morphology (captures reproducing aquatic hyphomycetes) and next generation sequencing (NGS; captures other fungi as well), on graded sizes of alder leaves (0.6 to 13.6 cm2). Leaves were submerged in two streams in geographically distant locations: the Oliveira Stream in Portugal and the Boss Brook in Canada. Decay rates of alder leaves and fungal sporulation rates did not differ between streams. Fungal biomass was higher in Boss Brook than in Oliveira Stream, and in both streams almost 100% of the reads belonged to active fungal taxa. In general, larger leaf areas tended to harbour more fungi, but these findings were not consistent between techniques. Morphospecies-based diversity increased with leaf area in Boss Brook, but not in Oliveira Stream; metabarcoding data showed an opposite trend. The higher resolution of metabarcoding resulted in steeper taxa-accumulation curves than morphospecies-based assessments (fungal conidia morphology). Fungal communities assessed by metabarcoding were spatially structured by leaf area in both streams. Metabarcoding promises greater resolution to assess biodiversity patterns in aquatic fungi and may be more accurate for assessing taxa-area relationships and local to global diversity ratios.
TypeArticle
URIhttp://hdl.handle.net/1822/50848
DOI10.1371/journal.pone.0181545
ISSN1932-6203
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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