Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/50371

TitleInternational Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - the quality controlled standard tool for routine identification of human and animal pathogenic fungi
Author(s)Irinyi, Laszlo
Serena, Carolina
Garcia-Hermoso, Dea
Arabatzis, Michael
Desnos-Ollivier, Marie
Vu, Duong
Cardinali, Gianluigi
Arthur, Ian
Sampaio, Paula
Pais, Célia
KeywordsDNA barcoding
ITS region
Fungal identification
Intraspecies/interspecies genetic diversity
Reference ITS database
intraspecies
interspecies genetic diversity
Issue dateMay-2015
PublisherOxford University Press
JournalMedical Mycology
Abstract(s)Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on "DNA barcoding of human and animal pathogenic fungi." A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.
Typearticle
URIhttp://hdl.handle.net/1822/50371
DOI10.1093/mmy/myv008
ISSN1369-3786
e-ISSN1460-2709
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:DBio - Artigos/Papers

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