Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/50370

TitleAssembling and auditing a comprehensive DNA barcode reference library for European marine fishes
Author(s)Oliveira, L. M.
Knebelsberger, T.
Landi, Monica
Soares, P.
Raupach, M. J.
Costa, Filipe O.
KeywordsAnimals
Europe
Fishes
Molecular Sequence Annotation
DNA Barcoding
Gene Library
Marine fishes
Reference library
Taxonomic reliability grade
Barcode
Index Number
Hidden diversity
Taxonomic
annotation protocol
Barcode Index Number
COI
Issue date2016
PublisherWiley
JournalJournal of Fish Biology
CitationOliveira L. M., Knebelsberger T., Landi M., Soares P. , Raupach M. J., Costa F. O. (2016). Assembling and auditing a global DNA barcode reference library for European marine fishes. Journal of Fish Biology 89: 2741–2754. doi:10.1111/jfb.13169.
Abstract(s)A large-scale comprehensive reference library of DNA barcodes for European marine fishes was assembled, allowing the evaluation of taxonomic uncertainties and species genetic diversity that were otherwise hidden in geographically restricted studies. A total of 4118 DNA barcodes were assigned to 358 species generating 366 Barcode Index Numbers (BIN). Initial examination revealed as much as 141 BIN discordances (more than one species in each BIN). After implementing an auditing and five-grade (A-E) annotation protocol, the number of discordant species BINs was reduced to 44 (13% grade E), while concordant species BINs amounted to 271 (78% grades A and B) and 14 other had insufficient data (grade D). Fifteen species displayed comparatively high intraspecific divergences ranging from 2·6 to 18·5% (grade C), which is biologically paramount information to be considered in fish species monitoring and stock assessment. On balance, this compilation contributed to the detection of 59 European fish species probably in need of taxonomic clarification or re-evaluation. The generalized implementation of an auditing and annotation protocol for reference libraries of DNA barcodes is recommended.
TypeArticle
URIhttp://hdl.handle.net/1822/50370
DOI10.1111/jfb.13169
ISSN2741–2754
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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