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TitleCharacterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma
Author(s)Scapulatempo-Neto, Cristovam
Veo, Carlos
Fregnani, José Humberto T.G.
Lorenzi, Adriana
Mafra, Allini
Melani, Armando G.F.
Loaiza, Edgar Antonio Alemán
Rosa, Luciana Albina Reis
Oliveira, Cristina Mendes de
Levi, José Eduardo
Longatto, Adhemar
KeywordsAnal carcinoma
Ki -67
Minichromosome maintenance protein 2
Topoisomerase II α
Human papillomavirus
Issue date31-Mar-2017
PublisherSpandidos Publications
JournalOncology Letters
CitationScapulatempo‑Neto, C., Veo, C., Fregnani, J. H. T., Lorenzi, A., Mafra, A., Melani, A. G., ... & Longatto‑Filho, A. (2017). Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma. Oncology Letters, 13(3), 1891-1898
Abstract(s)The present study aimed to ascertain the significance of topoisomerase II alpha (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the human papillomavirus (HPV) statuses of these lesions and characterize the immunohistochemical expression levels of TOP2A and MCM2 in anal carcinoma, as these are important markers for cervical HPV-induced lesions; their expression was also compared with respect to p16 and Ki-67. The vast majority of the cases tested positive for HPV16 (84%); 1 case tested positive for both HPV16 and HPV18. Positive HPV16 status was more frequent in early stages than in advanced stages (P=0.008). Positive immunohistochemical reactivity for MCM2 and TOP2A protein was observed in 71.6 and 100% of cases, respectively. Positive reactivity for p16 was significantly associated (P=0.001) with histological grade, and was more commonly expressed in squamous cell carcinoma than adenocarcinomas. HPV16 was strongly associated with positive p16 protein expression (76.6%). However, the high expression of Ki-67 combined with the high expression of p16 was predominantly observed in Stage III-IV cases. MCM2, TOP2A, p16 and Ki-67 exhibited intense positive staining in the anal lesions, indicating that these markers were significantly and constantly expressed in anal carcinoma.
Publisher version
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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