Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/49599
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Serrano, Joana B. | por |
dc.contributor.author | Martins, Filipa | por |
dc.contributor.author | Sousa, João Carlos | por |
dc.contributor.author | Pereira, Cátia D. | por |
dc.contributor.author | Pelt, Ans M. M. van | por |
dc.contributor.author | Rebelo, Sandra | por |
dc.contributor.author | Silva, Odete A. B. da Cruz e | por |
dc.date.accessioned | 2018-01-24T11:16:14Z | - |
dc.date.available | 2018-01-24T11:16:14Z | - |
dc.date.issued | 2017-04-07 | - |
dc.identifier.citation | Serrano, J. B., Martins, F., Sousa, J. C., Pereira, C. D., van Pelt, A. M., Rebelo, S., & da Cruz e Silva, O. A. (2017). Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis. Membranes, 7(2), 22. | por |
dc.identifier.issn | 2077-0375 | - |
dc.identifier.uri | https://hdl.handle.net/1822/49599 | - |
dc.description.abstract | Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time. The fact that LAP1 is expressed during nuclear elongation in spermiogenesis and is located at the spermatids' centriolar pole is singularly important. LAP1 binds to members of the protein phosphatase 1 (PP1) family. Similar localization of LAP1 and PP12, a testis-specific PP1 isoform, suggests a shared function for both proteins during spermiogenesis. Furthermore, this study suggests an involvement of LAP1 in manchette development and chromatin regulation possibly via interaction with acetylated-tubulin and lamins, respectively. Taken together, the present results indicate that, by moving to the posterior pole in spermatids, LAP1 can contribute to the achievement of non-random, sperm-specific chromatin distribution, as well as modulate cellular remodeling during spermiogenesis. In addition, LAP1 seems to be associated with dynamic microtubule changes related to manchette formation and flagella development. | por |
dc.description.sponsorship | Institute for Biomedicine (Project UID/BIM/04501/2013), the Fundação para a Ciência e Tecnologia of the Ministério da Educação e Ciência (Project PTDC/BEX-BCM/0493/2012), the COMPETE program (Programa Operacional Factores de Competitividade), the QREN (Quadro de Referência Estratégica Nacional) and the European Union (Fundo Europeu de Desenvolvimento Regional). Filipa Martins was the recipient of an FCT fellowship. The authors would also like to thank William Dauer (Columbia University, USA) for providing the anti-LAP1 antibody | por |
dc.language.iso | eng | por |
dc.publisher | MDPI Publishing | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147343/PT | por |
dc.rights | openAccess | por |
dc.subject | Nuclear envelope | por |
dc.subject | Lamina-associated polypeptide 1 | por |
dc.subject | Manchette | por |
dc.subject | Spermatids | por |
dc.subject | lamins | por |
dc.subject | protein phosphatase 1 | por |
dc.title | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/2077-0375/7/2/22/htm | por |
oaire.citationIssue | 22 | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2018-01-08T11:00:56Z | - |
dc.identifier.doi | 10.3390/membranes7020022 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Membranes | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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serrano et al 2016.pdf | 4,74 MB | Adobe PDF | Ver/Abrir |