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https://hdl.handle.net/1822/49093
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pinto, Vânia C. | por |
dc.contributor.author | Sousa, Paulo J. | por |
dc.contributor.author | Catarino, Susana Oliveira | por |
dc.contributor.author | Neves, Margarida Correia | por |
dc.contributor.author | Minas, Graça | por |
dc.date.accessioned | 2018-01-08T16:09:56Z | - |
dc.date.available | 2018-01-08T16:09:56Z | - |
dc.date.issued | 2017-04-15 | - |
dc.identifier.citation | Pinto, V., Sousa, P., Catarino, S. O., Correia-Neves, M., & Minas, G. (2017). Microfluidic immunosensor for rapid and highly-sensitive salivary cortisol quantification. Biosensors and Bioelectronics, 90, 308-313 | por |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://hdl.handle.net/1822/49093 | - |
dc.description.abstract | This paper presents a novel poly(dimethylsiloxane) (PDMS) microfluidic immunosensor that integrates a complementary metal-oxide-semiconductor (CMOS) optical detection system for a rapid and highly-sensitive quantification of salivary cortisol. The simple and non-invasive method of saliva sampling provides an interesting alternative to the blood, allowing a fast sampling at short intervals, relevant for many clinical diagnostic applications. The developed approach is based on the covalent immobilization of a coating antibody (Ab), a polyclonal anti-IgG, onto a treated PDMS surface. The coating Ab binds the capture Ab, an IgG specific for cortisol, allowing its correct orientation. Horseradish peroxidase (HRP)-labelled cortisol is added to compete with the cortisol in the sample, for the capture Ab binding sites. The HRP-labelled cortisol, bonded to the capture Ab, is measured through the HRP enzyme and the tetramethylbenzidine (TMB) substrate reaction. The cortisol quantification is performed by colorimetric detection of HRP-labelled cortisol, through optical absorption at 450 nm, using a CMOS silicon photodiode as the photodetector. Under the developed optimized conditions presented here, e.g., microfluidic channels geometry, immobilization method and immunoassay conditions, the immunosensor shows a linear range of detection between 0.01-20 ng/mL, a limit of detection (LOD) of 18 pg/mL and an analysis time of 35 min, featuring a great potential for point-of-care applications requiring continuous monitoring of the salivary cortisol levels during a circadian cycle. | por |
dc.description.sponsorship | FCT with the reference project UID/EEA/04436/2013, by FEDER funds through the COMPETE 2020 – Programa Operacional Competitividade e Internacionalização (POCI) with the reference project POCI-01-0145-FEDER-006941. V C Pinto thanks the FCT for the SFRH/BD/81526/2011 PhD grant. P J Sousa thanks the FCT for the SFRH/BD/81562/2011 PhD grant. S.O. Catarino thanks the FCT for the SFRH/BPD/108889/2015 grant, supported by national funds from Ministérios da Ciência, Tecnologia e Ensino Superior and by FSE through the POCH - Programa Operacional Capital Humano | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147325/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81526%2F2011/PT | por |
dc.rights | openAccess | por |
dc.subject | Microfluidic immunosensor | por |
dc.subject | Colorimetric detection | por |
dc.subject | Lab-on-a-chip (LOC) | por |
dc.subject | Poly(dimethylsiloxane) | por |
dc.subject | Salivary cortisol | por |
dc.title | Microfluidic immunosensor for rapid and highly-sensitive salivary cortisol quantification | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/journal/09565663 | por |
oaire.citationStartPage | 308 | por |
oaire.citationEndPage | 313 | por |
oaire.citationVolume | 90 | por |
dc.date.updated | 2017-12-19T16:28:06Z | - |
dc.identifier.doi | 10.1016/j.bios.2016.11.067 | por |
dc.identifier.pmid | 27931005 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biosensors and Bioelectronics | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals DEI - Artigos em revistas internacionais |
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untitled.pdf | 599,03 kB | Adobe PDF | Ver/Abrir |