Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/49032

TitleVasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?
Author(s)Castela, Angela
Gomes, Pedro
Silvestre, Ricardo Jorge Leal
Guardão, Luísa
Leite, Liliana
Chilro, Rui
Rodrigues, Ilda
Vendeira, Pedro
Virag, Ronald
Costa, Carla
KeywordsErectile Dysfunction
Diabetes
Endothelial Progenitor Cells
Insulin
Stromal Derived Factor-1 Alpha
Issue date2017
PublisherWiley
JournalJournal of Cellular Biochemistry
CitationCastela, A., Gomes, P., Silvestre, R., Guardão, L., Leite, L., Chilro, R., ... & Costa, C. (2017). Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?. Journal of cellular biochemistry, 118(1), 82-91
Abstract(s)Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1 and testosterone levels were evaluated by ELISA. Penile SDF-1 protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1 levels, which were significantly decreased in animals with diabetes. SDF-1 protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1 protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways.
TypeArticle
URIhttp://hdl.handle.net/1822/49032
DOI10.1002/jcb.25613
ISSN1097-4644
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.25613/full
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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