Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/48381

TitleNeospora caninum: high susceptibility to the parasite in C57BL/10ScCr mice
Author(s)Botelho, A. S.
Teixeira, L.
Costa, J. M. Correia da
Faustino, A. M. R.
Castro, António G.
Vilanova, M.
KeywordsNeospora caninum acute infection
Apicomplexa
C57BL/10 ScCr (ScCr) mice
C57BL/10 ScSn (ScSn) mice
NcT
Neospora caninum tachyzoites
IFN-γ, interferon-γ
IL-4, interleukin-4
IL-12, interleukin-12
TLR4, Toll-like receptor 4
IL-12R, IL-12 receptor
Th1, T helper 1
PBS, phosphate-buVered saline
RNA, ribonucleic acid
DNA, deoxyribonucleic acid
mRNA, messenger ribonucleic acid
cDNA, RNA to complementary DNA
PCR, polymerase chain reaction
HPRT, hypoxanthine phosphoribosyltransferase
dNTPs, deoxynucleotide triphosphate mix
interferon-gamma
interleukin-4
interleukin-12
toll-like receptor 4
IL-12 receptor
T helper 1
phosphate-buffered saline
ribonucleic acid
deoxyribonucleic acid
messenger ribonucleic acid
polymerase chain reaction
hypoxanthine phosphoribosyltransferase
deoxynucleotide triphosphate mix
cDNA
DNA
dNTPs
HPRT
IFN-?
IL-12
IL-12R
IL-4
interferon-?
mRNA
PBS
PCR
RNA
RNA to complementary DNA
Th1
TLR4
IFN-γ
interferon-γ
Issue date2007
PublisherElsevier
JournalExperimental Parasitology
Abstract(s)C57BL/10ScCr mice, lack Toll-like receptor 4 and a functional Interleukin-12 receptor. Taking this into account, susceptibility of these mice to Neospora caninum infection was assessed comparatively to that of immunocompetent C57BL/10ScSn mice. C57BL/10ScCr mice inoculated intraperitoneally with 5 x 10(5) N. caninum tachyzoites showed a high Susceptibility to this parasite. All infected C57BL/10ScCr mice were dead by day 8 post-infection whereas all control C57BL/10ScSn mice survived this parasitic challenge. Immunohistochemical analysis of infected C57BL/10ScCr mice showed N. caninum tachyzoites spread in the pancreas, liver, lung, intestine, heart and brain whereas no parasites were detected in similarly infected C57BL/10ScSn controls. The higher susceptibility of C57BL/10ScCr mice to neosporosis correlates with reduced interferon-gamma mRNA expression and increased IL-4 mRNA expression, comparatively to C57BL/10ScSn controls, detected in the spleen after the parasitic challenge. C57BL/10ScCr mice could thus be used as a new experimental model where to study immunobiological mechanisms associated with host Susceptibility to neosporosis.
TypeArticle
DescriptionNeospora caninum: high susceptibility to the parasite in C57BL/10ScCr mice
URIhttp://hdl.handle.net/1822/48381
DOI10.1016/j.exppara.2006.06.004
ISSN0014-4894
e-ISSN1090-244
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S0014489406001457
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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