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TitleMultilayered films produced by layer-by-layer assembly of chitosan and alginate as a potential platform for the formation of human adipose-derived stem cell aggregates
Author(s)Hatami, Javad
Silva, Sandra G.
Oliveira, Mariana Braga
Costa, Rui R.
Reis, R. L.
Mano, J. F.
Keywords3D culture
Layer by layer assembly
Multilayered film
Issue dateSep-2017
CitationHatami J., Silva S. G., Oliveira M. B., Costa R. R., Reis R. L., Mano J. F. Multilayered Films Produced by Layer-by-Layer Assembly of Chitosan and Alginate as a Potential Platform for the Formation of Human Adipose-Derived Stem Cell ..., Polymers, Vol. 9, Issue 9, pp. 440, doi:10.3390/polym9090440, 2017
Abstract(s)The construction of multilayered films with tunable properties could offer new routes to produce biomaterials as a platform for 3D cell cultivation. In this study, multilayered films produced with five bilayers of chitosan and alginate (CHT/ALG) were built using water-soluble modified mesyl and tosylâ CHT via layer-by-layer (LbL) self-assembly. NMR results demonstrated the presences of mesyl (2.83 ppm) and tosyl groups (2.39, 7.37 and 7.70 ppm) in the chemical structure of modified chitosans. The buildup of multilayered films was monitored by quartz-crystal-microbalance (QCM-D) and film thickness was estimated using the Voigt-based viscoelastic model. QCM-D results demonstrated that CHT/ALG films constructed using mesyl or tosyl modifications (mCHT/ALG) were significantly thinner in comparison to the CHT/ALG films constructed with unmodified chitosan (p < 0.05). Adhesion analysis demonstrated that human adipose stem cells (hASCs) did not adhere to the mCHT/ALG multilayered films and formed aggregates with sizes between ca. 100â 200 µm. In vitro studies on cell metabolic activity and live/dead staining suggested that mCHT/ALG multilayered films are nontoxic toward hACSs. Multilayered films produced via LbL assembly of ALG and off-the-shelf, water-soluble modified chitosans could be used as a scaffold for the 3D aggregates formation of hASCs in vitro.
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