Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/47929

TitleLaccase-mediated catechin grafting to chitosan for modulating antioxidant, antimicrobial activities and controlled release
Author(s)Kim, Suyeon
Nakamatsu, Javier
Torres, Fernando
Ribeiro, Artur
Gomes, Andreia
Cavaco-Paulo, Artur
Issue date13-Mar-2016
PublisherAmerican Chemical Society
JournalAbstracts of Papers of the American Chemical Society
CitationKim, Suyeon; Nakamatsu, Javier; Torres, Fernando; Ribeiro, Artur; Gomes, Andreia; Cavaco-Paulo, Artur, Laccase-mediated catechin grafting to chitosan for modulating antioxidant, antimicrobial activities and controlled release. 251st American Chemical Society National Meeting & Exposition - Computers in Chemistry. No. 612, San Diego, USA, March 13-17, 2016.
Abstract(s)In the present study, laccase from ascomycete myceliophthora thermophila was used for the simultaneous reactions of catechin oxidation to produce reactive o-quinions and subsequent reaction with free amino groups in chitosan (CS) through Schiff-base and Michael addition types reactions. These reactions were proved by UV-vis and ATR-IR spectroscopy showing the changes of peaks shape or characteristic new peaks appearances. The functionalised chitosan compounds, having a different degree of deacetylation (DDA) like 65, 95%, were further employed to film formations and showed different behaviours in swelling, erosion, thermal, and mechanical properties. The biological activities of antioxidant and antimicrobial were evaluated and showed the improvement of their activities by enzymatic grafting of strong antioxidant, catechin. The controlled release profiles of methylene blue (MB) and coomassie blue (CB) dyes from CS films were also studied and the DDA of CS, types of loading molecules, releasing medium, and the enzymatic grafting of CA were important parameters affecting release profiles of CS films.
TypeAbstract
Description251st American Chemical Society National Meeting & Exposition - Computers in Chemistry
URIhttp://hdl.handle.net/1822/47929
ISSN0065-7727
Publisher versionhttps://www.acs.org/content/acs/en/meetings/spring-2016.html
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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