Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/47659

TitleCarbon nanotubes accelerate methane production in pure cultures of methanogens and in a syntrophic coculture
Author(s)Salvador, Andreia F.
Martins, Gilberto
Melle-Franco, Manuel
Serpa, Ricardo
Stams, Alfons Johannes Maria
Cavaleiro, Ana Júlia
Pereira, M. Alcina
Alves, M. M.
Issue dateJul-2017
PublisherWiley-Blackwell
JournalEnvironmental Microbiology
CitationSalvador, Andreia F.; Martins, Gilberto; Melle-Franco, Manuel; Serpa, Ricardo; Stams, A. J. M.; Cavaleiro, Ana Júlia; Pereira, M. Alcina; Alves, M. Madalena, Carbon nanotubes accelerate methane production in pure cultures of methanogens and in a syntrophic coculture. Environmental Microbiology, 19(7), 2727-2739, 2017
Abstract(s)Carbon materials have been reported to facilitate direct interspecies electron transfer (DIET) between bacteria and methanogens improving methane production in anaerobic processes. In this work, the effect of increasing concentrations of carbon nanotubes (CNT) on the activity of pure cultures of methanogens and on typical fatty acid-degrading syntrophic methanogenic coculture was evaluated. CNT affected methane production by methanogenic cultures, although acceleration was higher for hydrogenotrophic methanogens than for acetoclastic methanogens or syntrophic coculture. Interestingly, the initial methane production rate (IMPR) by Methanobacterium formicicum cultures increased 17 times with 5 g·L1 CNT. Butyrate conversion to methane by Syntrophomonas wolfei and Methanospirillum hungatei was enhanced (1.5 times) in the presence of CNT (5 g·L1), but indications of DIET were not obtained. Increasing CNT concentrations resulted in more negative redox potentials in the anaerobic microcosms. Remarkably, without a reducing agent but in the presence of CNT, the IMPR was higher than in incubations with reducing agent. No growth was observed without reducing agent and without CNT. This finding is important to re-frame discussions and re-interpret data on the role of conductive materials as mediators of DIET in anaerobic communities. It also opens new challenges to improve methane production in engineered methanogenic processes. This article is protected by copyright. All rights reserved.
TypeArticle
URIhttp://hdl.handle.net/1822/47659
DOI10.1111/1462-2920.13774
ISSN1462-2912
e-ISSN1462-2920
Publisher versionhttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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