Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/47658

TitleSearching for new strategies against biofilm infections: Colistin-AMP combinations against Pseudomonas aeruginosa and Staphylococcus aureus single- and double-species biofilms
Author(s)Jorge, Paula
Grzywacz, Daria
Kamysz, Wojciech
Lourenço, Anália
Pereira, Maria Olívia
Issue date29-Mar-2017
PublisherPublic Library of Science
JournalPLoS ONE
CitationJorge, Paula; Grzywacz, Daria; Kamysz, Wojciech; Lourenço, Anália; Pereira, Maria Olívia, Searching for new strategies against biofilm infections: Colistin-AMP combinations against Pseudomonas aeruginosa and Staphylococcus aureus single- and double-species biofilms. PLoS One, 12(3), e0174654, 2017
Abstract(s)Antimicrobial research is being pressured to look for more effective therapeutics for the ever-growing antibiotic-resistant infections, and antimicrobial peptides (AMP) and antimicrobial combinations are promising solutions. This work evaluates colistin-AMP combinations against two major pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, encompassing non- and resistant strains. Colistin (CST) combined with the AMP temporin A (TEMP-A), citropin 1.1 (CIT-1.1) and tachyplesin I linear analogue (TP-I-L) was tested against planktonic, single- and double-species biofilm cultures. Overall synergy for planktonic P. aeruginosa and synergy/additiveness for planktonic S. aureus were observed. Biofilm growth prevention was achieved with synergy and additiveness. Pre-established 24 h-old biofilms were harder to eradicate, especially for S. aureus and double-species biofilms; still, some synergy and addictiveness was observed for higher concentrations, including for the biofilms of resistant strains. Different treatment times and growth media did not greatly influence AMP activity. CST revealed low toxicity compared with the other AMP but its combinations were toxic for high concentrations. Overall, combinations reduced effective AMP concentrations, mainly in prevention scenarios. Improvement of effectiveness and toxicity of therapeutic strategies will be further investigated.
TypeArticle
URIhttp://hdl.handle.net/1822/47658
DOI10.1371/journal.pone.0174654
ISSN1932-6203
e-ISSN1932-6203
Publisher versionhttp://journals.plos.org/plosone/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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