Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/47368

TítuloSynthesis of fusion genes for cloning by megaprimer-based PCR
Autor(es)Aguiar, Tatiana Quinta
Oliveira, Carla Cristina Marques de
Domingues, Lucília
Two-step PCR
Fusion genes
Molecular cloning
Restriction enzymes
Expression plasmids
RevistaMethods in Molecular Biology
CitaçãoAguiar, Tatiana Q.; Oliveira, Carla; Domingues, Lucília, Synthesis of fusion genes for cloning by megaprimer-based PCR. In Lucília Domingues (Ed.), PCR: Methods and Protocols, Vol. Methods in Molecular Biology 1620, New York: Springer, 2017. ISBN: 978-1-4939-7060-5, 101-112
Resumo(s)The polymerase chain reaction (PCR) is the technique of choice used to obtain DNA for cloning, because it rapidly provides high amounts of desired DNA fragments and allows the easy introduction of extremities adequate for enzyme restriction or homologous recombination, and of artificial, native, or modified sequence elements for specific applications. In this context, the use of megaprimer-based PCR strategies allows the versatile and fast assembly and amplification of tailor-made DNA sequences readily available for cloning. In this chapter, we describe the design and use of a megaprimer-based PCR protocol to construct customized fusion genes ready for cloning into commercial expression plasmids by restriction digestion and ligation.
Versão da editorahttps://link.springer.com/protocol/10.1007/978-1-4939-7060-5_16
Arbitragem científicayes
Aparece nas coleções:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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