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TitleRAPD/SCAR approaches for identification of adulterant breeds' milk in dairy products
Author(s)Cunha, Joana T.
Domingues, Lucília
KeywordsDNA extraction
Breed identification
Milk adulteration
Dairy authentication
Food quality control
Issue date2017
JournalMethods in Molecular Biology
CitationCunha, Joana T.; Domingues, Lucília, RAPD/SCAR approaches for identification of adulterant breeds' milk in dairy products. In Lucília Domingues (Ed.), PCR: Methods and Protocols, Vol. Methods in Molecular Biology 1620, New York: Springer, 2017. ISBN: 978-1-4939-7060-5, 183-193
Abstract(s)Food safety and quality are nowadays a major consumers concern. In the dairy industry the fraudulent addition of cheaper/lower-quality milks from nonlegitimate species/breeds compromises the quality and value of the final product. Despite the already existing approaches for identification of the species origin of milk, there is little information regarding differentiation at an intra-species level. In this protocol we describe a low-cost, sensitive, fast, and reliable analytical techniqueRandom Amplified Polymorphic DNA/Sequence Characterized Amplified Region (RAPD/SCAR)capable of an efficient detection of adulterant breeds in milk mixtures used for fraudulent manufacturing of dairy products and suitable for the detection of milk adulteration in processed dairy foods.
TypeBook part
Publisher version
AccessRestricted access (Author)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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