Please use this identifier to cite or link to this item:

TitleGelatin-based biodegradable ureteral stents for the treatment of urothelial tumors of the upper urinary tract cancer
Author(s)Barros, Alexandre António Antunes
Browne, S.
Oliveira, C.
Lima, Estêvão Augusto Rodrigues de
Duarte, Ana Rita C.
Healy, K. E.
Reis, R. L.
KeywordsCancer cells
Upper urinary tract
Issue dateDec-2016
PublisherMary Ann Liebert Inc.
JournalTissue Engineering. Part A
CitationBarros A. A., Browne S., Oliveira C., Lima E., Duarte A. R. C., Healy K. E., Reis R. L. Gelatin-based Biodegradable Ureteral Stents For The Treatment Of Urothelial Tumors Of The Upper Urinary Tract Cancer, Tissue Engineering Part A, Vol. 22, Issue S1, doi:10.1089/ten.tea.2016.5000.abstracts., 2016
Abstract(s)Upper urinary tract urothelial carcinoma (UTUC) accounts for 5â 10% of urothelial carcinomas and is a disease that has not been widely studied as carcinoma of the bladder. To avoid the problems of conventional therapies, such as the need for frequent drug instillation due to poor drug retention, we developed a biodegradable ureteral stent (BUS) impregnated by supercritical fluid CO2 (scCO2) with the most commonly used anti-cancer drugs, namely paclitaxel, epirubicin, doxorubicin, and gemcitabine. The release kinetics of anti-cancer therapeutics from drug-eluting stents was measured in artificial urine solution (AUS). The in vitro release showed a faster release in the first 72h for the four anti-cancer drugs, after this time a plateau was achieved and finally the stent degraded after 9 days. Regarding the amount of impregnated drugs by scCO2, gemcitabine showed the highest amount of loading (19.57â μg drug /mg polymer: 2% loaded), while the lowest amount was obtained for paclitaxel (0.067â μg drug /mg polymer: 0.01% loaded). A cancer cell line (T24) was exposed to graded concentrations (0.01 to 2000â ng/ml) of each drug for 4 and 72 hours to determine the sensitivities of the cells to each drug (IC50). The direct and indirect contact study of the anti-cancer biodegradable ureteral stents with the T24 and HUVEC cell lines confirmed the anti-tumoral effect of the BUS impregnated with the four anti-cancer drugs tested, reducing by 75% of the viability of the T24 cell line after 72h and demonstrating minimal cytotoxic effect on HUVECs.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Files in This Item:
File Description SizeFormat 
18981-ten_2Etea_2E2016_2E5000_2Eabstracts.pdf327,22 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID