Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/47096

TítuloGelatin-based biodegradable ureteral stents for the treatment of urothelial tumors of the upper urinary tract cancer
Autor(es)Barros, Alexandre António Antunes
Browne, S.
Oliveira, C.
Lima, Estêvão Augusto Rodrigues de
Duarte, Ana Rita C.
Healy, K. E.
Reis, R. L.
Palavras-chaveCancer cells
Drug-eluting
Stents
Upper urinary tract
DataDez-2016
EditoraMary Ann Liebert Inc.
RevistaTissue Engineering. Part A
CitaçãoBarros A. A., Browne S., Oliveira C., Lima E., Duarte A. R. C., Healy K. E., Reis R. L. Gelatin-based Biodegradable Ureteral Stents For The Treatment Of Urothelial Tumors Of The Upper Urinary Tract Cancer, Tissue Engineering Part A, Vol. 22, Issue S1, doi:10.1089/ten.tea.2016.5000.abstracts., 2016
Resumo(s)Upper urinary tract urothelial carcinoma (UTUC) accounts for 5â 10% of urothelial carcinomas and is a disease that has not been widely studied as carcinoma of the bladder. To avoid the problems of conventional therapies, such as the need for frequent drug instillation due to poor drug retention, we developed a biodegradable ureteral stent (BUS) impregnated by supercritical fluid CO2 (scCO2) with the most commonly used anti-cancer drugs, namely paclitaxel, epirubicin, doxorubicin, and gemcitabine. The release kinetics of anti-cancer therapeutics from drug-eluting stents was measured in artificial urine solution (AUS). The in vitro release showed a faster release in the first 72h for the four anti-cancer drugs, after this time a plateau was achieved and finally the stent degraded after 9 days. Regarding the amount of impregnated drugs by scCO2, gemcitabine showed the highest amount of loading (19.57â μg drug /mg polymer: 2% loaded), while the lowest amount was obtained for paclitaxel (0.067â μg drug /mg polymer: 0.01% loaded). A cancer cell line (T24) was exposed to graded concentrations (0.01 to 2000â ng/ml) of each drug for 4 and 72 hours to determine the sensitivities of the cells to each drug (IC50). The direct and indirect contact study of the anti-cancer biodegradable ureteral stents with the T24 and HUVEC cell lines confirmed the anti-tumoral effect of the BUS impregnated with the four anti-cancer drugs tested, reducing by 75% of the viability of the T24 cell line after 72h and demonstrating minimal cytotoxic effect on HUVECs.
TipoconferenceAbstract
URIhttp://hdl.handle.net/1822/47096
ISSN2152-4947
Versão da editorahttp://online.liebertpub.com/doi/full/10.1089/ten.tea.2016.5000.abstracts
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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