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|Title:||A semiautomated microfluidic platform for real-time investigation of nanoparticles' cellular uptake and cancer cells' tracking|
Maia, F. Raquel
Costa, Bruno Marques
Reis, R. L.
Oliveira, J. M.
Circulating tumor cells
|Publisher:||Future Medicine Ltd|
|Citation:||Carvalho M., Maia F. R., Silva-Correia J., Costa B. M., Reis R. L., Oliveira J. M. A semiautomated microfluidic platform for real-time investigation of nanoparticles' cellular uptake and cancer cells' tracking., Nanomedicine, doi:10.2217/nnm-2016-0344, 2017|
|Abstract(s):||Aims: develop a platform composed of labeled dendrimer nanoparticles and a microfluidic device for real-time monitoring of cancer cells fate. Materials and Methods: The physicochemical and biological characterization of the developed Carboxymethyl-chitosan/poly(amidoamine) (CMCht/PAMAM) dendrimer nanoparticles were performed using TEM, AFM, Zeta Sizer, DSC and cytotoxicity screening. Cancer cell lines derived from different tumor types, including HeLa (Cervical Carcinoma), HCT-116 (Colon Carcinoma) and U87MG (Glioblastoma), were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 3 days (MTS/DNA). Results: Nanoparticles were successfully modified with an average size of 50 nm. Internalization levels go from 87% to 100% in static and from 95% to 100% in dynamic conditions. Viability levels range from 95% to 100% in static and from 90% to 100% in dynamic conditions, being HCT the most sensitive to the presence of the NP. Conclusions: the results show different responses to the presence of 0.5 mg.mL-1 dendrimer nanoparticles when comparing static to dynamic conditions, with a tendency towards higher sensitivity when subjected to confinement. This work demonstrated that the proposed microfluidic-based platform allows real-time cell monitoring, which, upon more studies, namely the assessment of the drug release effect, could be used for cancer theranostics.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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