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TitleNatural based eumelanin nanoparticles functionalization and preliminary evaluation as carrier for gentamicin
Author(s)De Trizio, Antonella
Srisuk, Pathomthat
Costa, Rui R.
Fraga, Alexandra G.
Modena, Tiziana
Genta, Ida
Dorati, Rossella
Pedrosa, Jorge
Conti, Bice
Correlo, V. M.
Reis, R. L.
KeywordsDrug delivery systems
Eumelanin nanoparticles
Surface modification
Eumelanin nanopartides
Issue dateMar-2017
PublisherElsevier B.V.
JournalReactive and Functional Polymers
CitationDe Trizio A., Srisuk P., Costa R. R., Fraga A. G., Modena T., Genta I., Dorati R., Pedrosa J., Conti B., Correlo V. M., Reis R. L. Natural based eumelanin nanoparticles functionalization and preliminary evaluation as carrier for gentamicin, Reactive & Functional Polymers, Vol. 114, pp. 38-48, doi:10.1016/j.reactfunctpolym.2017.03.004, 2017
Abstract(s)Purpose of the work was to modified natural based eumelanin nanoparticles surface by dopamine selfpolymerization (FEUNp), facilitating conjugation through polydopamine bioactive functional groups and improving nanoparticle surface hydrophilicity. SEM, TEM, and AFM characterization confirmed FEUNp spherical shape (230.04 ± 8.25 nm) and their polydopamine coating. Individual indole or indoline structure spectrum at 1600 cm−1 and C:N mass 9.08 was highlighted by FTIR and XPS analysis respectively. Quartzcrystal microbalance with dissipation monitoring (QCM-D) and thermal gravimetric analysis (TGA) showed successful polydopamine adsorption to eumelanin nanoparticles surface, a weight ratio of eumelanin nanoparticles/dopamine of 1/2.46 in the wet state and 1/0.20 in the dry state; the −36.60 ± 0.45 mV negatively surface charges confirms the presence of PD covering. FEUNp were loaded with gentamicin sulfate, for application in infectious diseases therapies, such as osteomyelitis. Nanoparticles drug entrapment efficiency was 32.42 ± 3.21%, and ζ-potential close to neutrality (−1.84 ± 0.58 mV). FEUNp-GS antimicrobial effect was tested on Staphylococcus aureus and Escherichia coli showing gentamicin 24 h sustained release from FEUNp-GS. IC50 and IC90 were 110.60 μg/mL and 216.39 μg/mL against S. aureus, and 54.13 μg/mL and 101.25 μg/mL against E. coli. The results are promising for FEUNp-GS as delivery system potentially useful diverse administration routes.
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Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals
ICVS - Artigos em revistas internacionais / Papers in international journals

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