Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/46862

TitleScreening of nanocomposite scaffolds arrays using superhydrophobic-wettable micropatterns
Author(s)Leite, Álvaro J.
Oliveira, Mariana Braga
Caridade, Sofia Glória Ferreira
Mano, J. F.
KeywordsArray-based screening
Combinatorial biomaterials screening
Flat platforms
High-throughput screening
Nanocomposites
Nondestructive image analysis
Issue dateMay-2017
PublisherWiley-VCH Verlag
JournalAdvanced Functional Materials
CitationLeite A. J., Oliveira M.B., Caridade S. G., Mano J. F. Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns, Advanced Functional Materials, Vol. -, Issue -, pp. -, doi:10.1002/adfm.201701219, 2017
Abstract(s)Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments.
TypeArticle
URIhttp://hdl.handle.net/1822/46862
DOI10.1002/adfm.201701219
ISSN1616-3028
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/adfm.201701219/abstract
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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