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dc.contributor.authorMhanna, Ramipor
dc.contributor.authorBecher, Janapor
dc.contributor.authorSchnabelrauch, Matthiaspor
dc.contributor.authorReis, R. L.por
dc.contributor.authorPashkuleva, I.por
dc.date.accessioned2017-10-26T17:22:42Z-
dc.date.issued2017-06-
dc.date.submitted2017-06-
dc.identifier.citationMhanna R., Becher J., Schnabelrauch M., Reis R. L., Pashkuleva I. Sulfated alginate as a mimic of sulfated glycosaminoglycans: Binding of growth factors and effect on stem cell behavior, Advanced Biosystems, Vol. 1, Issue 7, pp. 1700043, doi:10.1002/adbi.201700043, 2017por
dc.identifier.issn2366-7478por
dc.identifier.urihttp://hdl.handle.net/1822/46859-
dc.description.abstractSulfated glycosaminoglycans (GAGs) are principal elements of the extracellular matrix, where they are involved in a plethora of signaling pathways mainly via interactions with diverse proteins such as growth factors and cytokines. However, the mechanisms that drive these interactions are not yet clear, mostly because of the difficulty to access large quantities of homogeneously sulfated natural GAGs. In this work, GAG mimics are synthesized from readily available alginate with different degrees of sulfation (DS, from 0.8 to 2.6) by simple process. The effect of the DS is determined on the binding of basic fibroblast growth factor (FGF-2). The enzyme-linked immunosorbent assay demonstrates that the binding of FGF-2 is significantly greater for alginates with high DS as compared to unmodified and low sulfated analogs. These results are further applied to engineer FGF-2-loaded substrates for stem cell culturing using the layer-by-layer approach. All films support the attachment and growth of adipose derived stem cells (ADSCs). Noteworthy, highly sulfated alginates maintain the stemness of the ADSCs that exhibit remarkably long filopodia. These results can be exploited in the engineering of novel substrates that induce targeted cell behavior via controlled protein delivery and for tissue engineering constructs applicable in various regenerative approaches.por
dc.description.sponsorshipThe authors acknowledge the EU (ERC-2012-AdG-20120216-321266 ComplexiTE and H2020-TWIN-CHEM2NATURE-692333) and the Portuguese FCT (IF/00032/2013; ENMed/0001/2015). The authors also thank the Lebanese National Council for Scientific Research (CNRS) and the University Research Board at the American University of Beirut.por
dc.language.isoengpor
dc.publisherWiley-VCH Verlagpor
dc.relationERC-2012-AdG-20120216-321266por
dc.relationH2020-TWIN-CHEM2NATURE-692333por
dc.relationIF/00032/2013por
dc.relationENMed/0001/2015por
dc.rightsrestrictedAccesspor
dc.subjectAlginatepor
dc.subjectBiomimetic materialspor
dc.subjectGrowth factorspor
dc.subjectLayer-by-layerpor
dc.subjectSulfated glycosaminoglycanspor
dc.titleSulfated alginate as a mimic of sulfated glycosaminoglycans: binding of growth factors and effect on stem cell behaviorpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/adbi.201700043/epdfpor
dc.commentshttp://3bs.uminho.pt/node/19111por
degois.publication.firstPage1700043por
degois.publication.issue7por
degois.publication.volume1por
dc.date.updated2017-09-25T14:45:56Z-
dc.identifier.doi10.1002/adbi.201700043por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
sdum.journalAdvanced Biosystemspor
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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