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TitleSelf-assembled hydrogel fiber bundles from oppositely charged polyelectrolytes mimic micro-/nanoscale hierarchy of collagen
Author(s)Sant, Shilpa
Coutinho, Daniela F.
Gaharwar, Akhilesh K.
Neves, N. M.
Reis, R. L.
Gomes, Manuela E.
Khademhosseini, Ali
KeywordsBottom-up self-assembly
Chitosan and gellan gum
Collagen mimicking
Hierarchical hydrogel fibers
Polyelectrolyte Complexes
Issue dateAug-2017
PublisherWiley-VCH Verlag
JournalAdvanced Functional Materials
CitationSant S., Coutinho D. F., Gaharwar A. K., Neves N. M., Reis R. L., Gomes M. E., Khademhosseini A. Self-Assembled Hydrogel Fiber Bundles from Oppositely Charged Polyelectrolytes Mimic Micro-/Nanoscale Hierarchy of Collagen, Adv. Funct. Mater., doi:10.1002/adfm.201606273, 2017
Abstract(s)Fiber bundles are present in many tissues throughout the body. In most cases, collagen subunits spontaneously self-assemble into a fibrilar structure that provides ductility to bone and constitutes the basis of muscle contraction. Translating these natural architectural features into a biomimetic scaffold still remains a great challenge. Here, a simple strategy is proposed to engineer biomimetic fiber bundles that replicate the self-assembly and hierarchy of natural collagen fibers. The electrostatic interaction of methacrylated gellan gum with a countercharged chitosan polymer leads to the complexation of the polyelectrolytes. When directed through a polydimethylsiloxane channel, the polyelectrolytes form a hierarchical fibrous hydrogel demonstrating nanoscale periodic light/dark bands similar to D-periodic bands in native collagen and align parallel fibrils at microscale. Importantly, collagen-mimicking hydrogel fibers exhibit robust mechanical properties (MPa scale) at a single fiber bundle level and enable encapsulation of cells inside the fibers under cell-friendly mild conditions. Presence of carboxyl- (in gellan gum) or amino- (in chitosan) functionalities further enables controlled peptide functionalization such as Arginylglycylaspartic acid (RGD) for biochemical mimicry (cell adhesion sites) of native collagen. This biomimetic-aligned fibrous hydrogel system can potentially be used as a scaffold for tissue engineering as well as a drug/gene delivery vehicle.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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