Please use this identifier to cite or link to this item:

TitleMeasurement of acetate during the production of recombinant proteins with E. coli
Author(s)Rocha, I.
Ferreira, E. C.
Issue date5-Oct-2000
CitationINTERNATIONAL CONFERENCE RECOMBINANT PROTEIN PRODUCTION WITH PROKARYOTIC AND EUKARYOTIC HOST CELLS - " International Conference Recombinant Protein Production with prokaryotic and eukaryotic host cells". Semmering: [s.n.], 2000.
Abstract(s)Acetate is known to be the major by-product during fermentation of recombinant E. coli. Among the consequences of that production, the more important are the decrease of the biomass yield for a given carbon source, the inhibition of the growth when acetate is present at high concentrations (typically 10 g/L) and the decrease of the production of recombinant proteins. For these reasons, its accurate and fast measurement is a very important issue when trying to achieve high productivities in this kind of processes. A method based on Flow Injection Analysis (FIA) was then adapted and optimized for the on-line monitoring of fermentations of recombinant E. coli. The method is based on the pre-acidification of the sample with sulfuric acid followed by the diffusion of the acetate into a stream containing an acid-base indicator through a hydrophobic membrane. The decrease in the absorbance of the acid-base indicator at 560 nm is proportional to the concentration of acetate and is measured with a photometer for that wavelength. The composition of the indicator was studied in order to achieve a compromise between stability and sensitivity of the method. Several solutions are proposed, depending on the concentration range of acetate to be measured. It was possible to achieve linearity until 10 g/L of acetate with a sensibility of less than 0.1 g/L. The correlation between acetate measured with FIA and with other methods (HPLC and enzymatic kit from R-Biopharm) is also acceptable, the differences never exceeding 20%. The method is very reproducible, being the averaged relative standard deviations around 2% for 20 replicates of the same sample. The method is also very fast, being the sampling rate of 30 hˉ¹.
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

Files in This Item:
File Description SizeFormat 
4.7.28.Semmering2000-RESUMO[1].pdfResumo7,11 kBAdobe PDFView/Open
4.7.28.Semmering2000-POSTER[1].pdfPoster97,22 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID