Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/46659

TitleUltrasound-assisted swelling of bacterial cellulose
Author(s)Song, J.
Su, Jing
Loureiro, Ana
Sá, M.
Cavaco-Paulo, Artur
Kim, Hye Rim
Silva, Carla
KeywordsBacterial cellulose
PEG-FITC
Polymerization
Swelling
Ultrasound
Issue dateOct-2017
PublisherWiley-Blackwell
JournalEngineering in Life Sciences
CitationSong, J.; Su, Jing; Loureiro, Ana; Sá, M.; Cavaco-Paulo, Artur; Kim, Hye Rim; Silva, Carla, Ultrasound-assisted swelling of bacterial cellulose. Engineering in Life Sciences, 17(10), 1108-1117, 2017
Abstract(s)Bacterial cellulose (BC) was obtained by static cultivation using commercial BC gel from scoby. BC membranes (oven dried and freeze-dried) were swelled with 8% NaOH, in absence and in presence of ultrasound (US), for 30, 60 and 90 min. The influence of swelling conditions on both physico-chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30 min. at room temperature in the presence of US. Native and PEGylated laccase from Myceliophthora Thermophila was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5-7 U/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1-2 U/gBC). BC swelled membranes are presented herein as a potential support for the preparation of immobilized enzymes for industrial applications, like phenolics polymerization.
TypeArticle
URIhttp://hdl.handle.net/1822/46659
DOI10.1002/elsc.201700085
ISSN1618-0240
e-ISSN1618-2863
Publisher versionhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1618-2863
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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