Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/46343

TitleBiofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles
Author(s)Batistão, D.
Amaral de Campos, Paola
Camilo, Nayara Caroline
Royer, Sabrina
Araújo, Bruna Fuga
Naves, Karinne Spirandelli Carvalho
Martins, M.
Pereira, Maria Olívia
Henriques, Mariana
Gontijo-Filho, Paulo Pinto
Botelho, Cláudia M.
Oliveira, Rosário
Ribas, Rosineide Marques
Issue dateApr-2016
PublisherSociety for General Microbiology
JournalJournal of Medical Microbiology
CitationBatistão, D.; Amaral de Campos, Paola; Camilo, Nayara Caroline; Royer, Sabrina; Araújo, Bruna Fuga; Naves, Karinne Spirandelli Carvalho; Martins, M.; Pereira, Maria Olívia; Henriques, Mariana; Gontijo-Filho, Paulo Pinto; Botelho, Cláudia M.; Oliveira, Rosário; Ribas, Rosineide Marques, Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles. Journal of Medical Microbiology, 65(4), 286-297, 2016
Abstract(s)Biofilms plays an important role in medical-device-related infections. This study aimed to determine the factors that influence adherence and biofilm production, as well as the relationship between strong biofilm production and genetic determinants in clinical isolates of meticillin-resistant Staphylococcus aureus (MRSA). Fifteen strains carrying different chromosomal cassettes recovered from hospitalized patients were selected; five SCCmecII, five SCCmecIII and five SCCmecIV. The SCCmec type, agr group and the presence of the virulence genes (bbp, clfA, icaA, icaD, fnbB, bap, sasC and IS256) were assessed by PCR. PFGE and multilocus sequence typing (MLST) techniques were also performed. The initial adhesion and biofilm formation were examined by quantitative assays. The surface tension and hydrophobicity of the strains were measured by the contact angle technique to evaluate the association between these parameters and adhesion ability. SCCmecIII and IV strains were less hydrophilic, with a high value for the electron acceptor parameter and higher adhesion in comparison with SCCmecII strains. Only SCCmecIII strains could be characterized as strong biofilm producers. The PFGE showed five major pulsotypes (AE); however, biofilm production was related to the dissemination of one specific PFGE clone (C) belonging to MLST ST239 (Brazilian epidemic clonal complex). The genes agrI, fnbB and IS256 in SCCmecIII strains were considered as genetic determinants associated with strong biofilm-formation by an ica-independent biofilm pathway. This study contributes to the understanding of biofilm production as an aggravating factor potentially involved in the persistence and severity of infections caused by multidrug-resistant MRSA belonging to this genotype.
TypeArticle
URIhttp://hdl.handle.net/1822/46343
DOI10.1099/jmm.0.000228
ISSN0022-2615
Publisher versionhttp://jmm.microbiologyresearch.org/content/journal/jmm
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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