Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/46277

TitleDetecting antibody-labeled BCG MNPs using a magnetoresistive biosensor and magnetic labeling technique
Author(s)Barroso, Teresa R. G.
Martins, Verónica C.
Cardoso, Filipe
Cardoso, Susana
Pedrosa, Jorge
Neves, Margarida Correia
Rivas, José
Freitas, P. P.
KeywordsTuberculosis
Nanotechnology
Diagnosis
Sandwich-immunoassay
Magnetic nanoparticles
Magnetoresistive biochip
Issue date2015
PublisherTrans Tech Publications
JournalJournal of Nano Research
Abstract(s)Tuberculosis is still a major global health concern, causing the estimated death of 1.5 million people per year and being associated with high morbidity. The development of point-of-care diagnostic tools for tuberculosis is mandatory, especially because the fast and accurate detection of the slow-growing Mycobacterium tuberculosis by the conventional diagnostic tests is difficult. The objective of this work was to develop the first steps to achieve a portable method for the diagnosis of tuberculosis, by a sandwich-immunoassay combined with magnetoresistive biochip technology. With the purpose of conjugating 250 nm streptavidin-coated magnetic nanoparticles with anti-M. tuberculosis biotinylated antibodies, Mycobacterium bovis Bacillus Calmette-Guerin was used as a surrogate for M. tuberculosis bacteria. After magnetic capture, target bacteria were brought in contact with the surface of the magnetoresistive biochip previously functionalized with a secondary anti-M. tuberculosis antibody. Magnetically labeled cells were detected by an array of spin-valve sensors, which change their electrical resistance in the presence of the fringe field of the magnetic particles. Optimization studies on the efficiency of the magnetic capture and further recognition of the bacteria by the secondary antibody on the biochip surface were conducted. The results on the magnetoresistive biochip showed a clear difference in the signal between specific and control ( nonspecific) sensors, suggesting the usefulness of this technique as a potential biorecognition tool for the development of a point-of-care diagnostic method for tuberculosis.
TypeArticle
URIhttp://hdl.handle.net/1822/46277
DOI10.4028/www.scientific.net/JNanoR.34.49
ISSN1661-9897
Publisher versionhttp://www.scientific.net/JNanoR/details
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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