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TitleDODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system
Author(s)Carneiro, Catarina
Correia, Alexandra
Collins, Tony
Vilanova, Manuel
Pais, Célia
Gomes, Andreia
Real Oliveira, M. Elisabete C. D.
Sampaio, Paula
KeywordsAdjuvants, Immunologic
Candida albicans
Cell Wall
Drug Delivery Systems
DODAB:MO liposomes
Adjuvant activity
Antigen delivery systems
Candida albicans proteins
Liposome endocytosis
Humoral and cellular immune responses
Antigen protection
Issue dateJan-2015
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
CitationCarneiro C., Correia A., Collins T., Vilanova M., Pais C., Gomes A.C., Real Oliveira M.E.C.D. and Sampaio P. (2015). DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system. European Journal of Pharmaceutics and Biopharmaceutics, 89(2015),190–200. doi: 10.1016/j.ejpb.2014.11.028.
Abstract(s)We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 ± 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly taken up by macrophage cells and accumulated in membrane rich regions with an internalization time of 20 min. Immunized mice displayed strong humoral and cell-mediated immune responses, producing antibodies (IgGs) against specific cell wall proteins, Cht3p and Xog1p. DODAB:MO-based liposomes loaded with C. albicans proteins have an excellent immunogenic potential and can be explored for the development of an immunoprotective strategy against Candida infections.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CDF - FAMO - Artigos/Papers (with refereeing)
DBio - Artigos/Papers

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