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|Title:||Osteogenesis evaluation of duck’s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone|
|Author(s):||Kook, Y. J.|
Lee, D. H.
Song, J. E.
Jeon, Y. S.
Oliveira, J. M.
Reis, R. L.
Duck's feet-derived collagen
|Publisher:||BioMed Central (BMC)|
|Citation:||Kook Y. J., Lee D. H., Song J. E., Tripathy N., Jeon Y. S., Oliveira J. M., Reis R. L., Khang G. Osteogenesis evaluation of duck’s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone, Biomaterials Research, Vol. 21, Issue 2, pp. 1-7, doi:10.1186/s40824-017-0088-4, 2017|
|Abstract(s):||Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(â )DC and Dex(â )-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(â )DC, Dex(â )-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR). Results: In this study, we assessed osteogenic differentiation of BMSCs on Duckâ s feet-derived collagen (DC)/ HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days. Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|
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