Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/45013

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dc.contributor.authorCunha, M. N. M.por
dc.contributor.authorFelgueiras, Helena Pradopor
dc.contributor.authorGouveia, Isabelpor
dc.contributor.authorZille, Andreapor
dc.date.accessioned2017-03-15T11:26:24Z-
dc.date.available2017-03-15T11:26:24Z-
dc.date.issued2017-
dc.identifier.issn0927-7765por
dc.identifier.urihttp://hdl.handle.net/1822/45013-
dc.description"In Press, Accepted Manuscript, Available online 12 March 2017"por
dc.description.abstractSilver nanoparticles (AgNPs) were synthesized by citrate reduction method in the presence of polymers, poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA) and chitosan, used as stabilizing agents, and an oxidoreductase enzyme, laccase (Lac), with the goal of expanding the NPs antimicrobial action. AgNPs were characterized by UV-visible spectrometry, dynamic light scattering and transmission electron microscopy. As protecting agents, PEG and PVA promoted the formation of spherical uniformly-shaped, small-sized, monodispersed AgNPs (≈ 20 nm). High Mw polymers were established as most effective in producing small-sized NPs. Chitosan's viscosity led to the formation of aggregates. Despite the decrease in Lac activity registered for the hybrid formulation, AgNPs-polymer-Lac, a significant augment in stability over time (up to 13 days, at 50 °C) was observed. This novel formulation displays improved synergistic performance over AgNPs-Lac or polymer-Lac conjugates, since in the former the Lac activity becomes residual at the end of 3 days. By enabling many ionic interactions, chitosan restricted the mass transfer between Lac and substrate and, thus, inhibited the enzymatic activity. These hybrid nanocomposites made up of inorganic NPs, organic polymers and immobilized antimicrobial oxidoreductive enzymes represent a new class of materials with improved synergistic performance. Moreover, the Lac and the AgNPs different antimicrobial action, both in time and mechanism, may also constitute a new alternative to reduce the probability of developing resistance-associated mutations.por
dc.description.sponsorshipThis work was funded by Portuguese Foundation for Science and TechnologyFCT/MCTES (PIDDAC) and co-financed by European funds (FEDER) through the PT2020 program, research projectM-ERA-NET/0006/2014. A. Zille and H. P. Felgueiras also acknowledge funding from FCT within the scope of the project POCI-01-0145-FEDER-007136 and UID/CTM/00264.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147331/PTpor
dc.rightsopenAccesspor
dc.subjectSilver nanoparticlespor
dc.subjectEnzymatic stabilitypor
dc.subjectLaccasepor
dc.subjectSynergistic effectpor
dc.subjectPolymer stabilizerpor
dc.titleSynergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymerspor
dc.typearticlepor
dc.peerreviewedyespor
sdum.publicationstatusinfo:eu-repo/semantics/publishedVersionpor
oaire.citationStartPage210por
oaire.citationEndPage220por
oaire.citationTitleColloids and Surfaces B: Biointerfacespor
oaire.citationVolume154por
dc.identifier.doi10.1016/j.colsurfb.2017.03.023por
dc.identifier.pmid28343119por
dc.subject.fosEngenharia e Tecnologia::Nanotecnologiapor
dc.subject.wosScience & Technologypor
sdum.journalColloids and Surfaces B: Biointerfacespor
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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