Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/44806

TítuloNitrogen and carbon source balance determined longevity, independently of fermentative or respiratory metabolism in the yeast Saccharomyces cerevisiae
AutorSantos, Júlia
Correia, Fernanda Leitão
Sousa, Maria João
Leão, Cecília
Data2016
CitaçãoSantos, J., Leitão-Correia, F., Sousa, M. J., & Leão, C. (2016). Nitrogen and carbon source balance determines longevity, independently of fermentative or respiratory metabolism in the yeast Saccharomyces cerevisiae. - 7(- 17), - 23042. doi: 10.18632/oncotarget.8656
ResumoDietary regimens have proven to delay aging and age-associated diseases in several eukaryotic model organisms but the input of nutritional balance to longevity regulation is still poorly understood. Here, we present data on the role of single carbon and nitrogen sources and their interplay in yeast longevity. Data demonstrate that ammonium, a rich nitrogen source, decreases chronological life span (CLS) of the prototrophic Saccharomyces cerevisiae strain PYCC 4072 in a concentration-dependent manner and, accordingly, that CLS can be extended through ammonium restriction, even in conditions of initial glucose abundance. We further show that CLS extension depends on initial ammonium and glucose concentrations in the growth medium, as long as other nutrients are not limiting. Glutamine, another rich nitrogen source, induced CLS shortening similarly to ammonium, but this effect was not observed with the poor nitrogen source urea. Ammonium decreased yeast CLS independently of the metabolic process activated during aging, either respiration or fermentation, and induced replication stress inhibiting a proper cell cycle arrest in G0/G1 phase. The present results shade new light on the nutritional equilibrium as a key factor on cell longevity and may contribute for the definition of interventions to promote life span and healthy aging.
Tipoarticle
URIhttp://hdl.handle.net/1822/44806
DOI10.18632/oncotarget.8656
ISSN1949-2553
Versão da editorahttp://www.impactjournals.com/oncotarget/
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee

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