Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/44749

TitleInflammatory response to dextrin-based hydrogel associated with human mesenchymal stem cells, urinary bladder matrix and Bonelike ® granules in rat subcutaneous implants
Author(s)Silva, D.
Caseiro, Ana Rita
Amorim, Irina
Pereira, Isabel
Faria, Fátima
Pereira, Tiago
Santos, José Domingos
Gama, F. M.
Maurício, Ana Colette
Keywordsbiocompatibility
dextrin hydrogel
human mesenchymal stem cells
inflammatory response
injectable matrix
Issue date27-Oct-2016
PublisherIOP Publishing
JournalBiomedical Materials
CitationSilva, D.; Caseiro, Ana Rita; Amorim, Irina; Pereira, Isabel; Faria, Fátima; Pereira, Tiago; Santos, José Domingos; Gama, F. M.; Maurício, Ana Colette, Inflammatory response to dextrin-based hydrogel associated with human mesenchymal stem cells, urinary bladder matrix and Bonelike ® granules in rat subcutaneous implants. Biomedical Materials (Bristol, England), 11(6), 65004, 2016
Abstract(s)Increasing relevance has been attributed to hydrogels due to their ability to provide an extracellular matrix (ECM)-like environment for cellular adhesion and proliferation, acting as mechanical scaffolds for tissue remodeling or as delivery matrices. In vivo biocompatibility of a hybrid dextrin hydrogel produced from oxidized dextrin and adipic acid dihydrazide and its combinations with human mesenchymal stem cells (hMSCs), ECM from a porcine bladder (urinary bladder matrix) and ceramic granules (Bonelike ® ), was evaluated following ISO 10993 after subcutaneous implantation in a rat model. Histological analysis after 3 and 15 d showed typical acute and chronic inflammatory responses, respectively, with a more severe reaction exhibited whenever the ceramic granules were present. However, the dextrin hydrogel was able to stabilize granules in the implant site. Dextrin hydrogel was scored as slight irritant after 3 d, similar to its combination with UBM, and as non-irritant after 15 d. The presence of viable hMSCs in the subcutaneous tissue could be confirmed by the presence of anti-human nuclei antibody (HuNu + ) cells. The production of growth factors and inflammatory and immunomodulatory cytokines by these cells was also quantified in peripheral blood confirming the successful encapsulation of hMSCs into the hydrogel matrix for cell survival promotion. The presence of hMSCs seemed to modulate the inflammatory response by accelerating its progression when compared to the acellular experimental groups. Dextrin hydrogel has proven to be a biocompatible multifunctional matrix for minimally invasive biomedical procedures, including orthopedic surgeries when associated with bone substitutes and also as a possible encapsulation matrix for cell-based therapies.
TypeArticle
URIhttp://hdl.handle.net/1822/44749
DOI10.1088/1748-6041/11/6/065004
ISSN1748-6041
e-ISSN1748-605X
Publisher versionhttp://iopscience.iop.org/journal/1748-605X
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_46511_1.pdf
  Restricted access
14,23 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID