Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/44734

TitleThaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
Author(s)Marani, Mariela M.
Perez, Luis O.
Araujo, Alyne Rodrigues de
Plácido, Alexandra
Sousa, Carla F.
Quelemes, Patrick Veras
Oliveira, Mayara
Alves, Ana G. Gomes
Pueta, Mariana
Gameiro, Paula
Tomás, Ana M.
Delerue-Matos, Cristina
Eaton, Peter
Camperi, Silvia A.
Basso, Néstor G.
Leite, Jose Roberto de Souza de Almeida
KeywordsAnura
Atomic force microscopy
cDNA
Circular dichroism
Surface Plasmon Resonance
Issue date20-Mar-2017
PublisherElsevier
JournalGene
CitationMarani, Mariela M.; Perez, Luis O.; Araujo, Alyne Rodrigues de; Plácido, Alexandra; Sousa, Carla F.; Quelemes, Patrick Veras; Oliveira, Mayara; Alves, A.; Pueta, Mariana; Gameiro, Paula; Tomás, Ana M.; Delerue-Matos, Cristina; Eaton, Peter; Camperi, Silvia A.; Basso, Néstor G.; Leite, Jose Roberto de Souza de Almeida, Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli. Gene, 605, 70-80, 2017
Abstract(s)Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 M). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.
TypeArticle
URIhttp://hdl.handle.net/1822/44734
DOI10.1016/j.gene.2016.12.020
ISSN0378-1119
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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