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|Title:||Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli|
|Author(s):||Marani, Mariela M.|
Perez, Luis O.
Araujo, Alyne Rodrigues de
Sousa, Carla F.
Quelemes, Patrick Veras
Alves, Ana G. Gomes
Tomás, Ana M.
Camperi, Silvia A.
Basso, Néstor G.
Leite, Jose Roberto de Souza de Almeida
Atomic force microscopy
Surface Plasmon Resonance
|Citation:||Marani, Mariela M.; Perez, Luis O.; Araujo, Alyne Rodrigues de; Plácido, Alexandra; Sousa, Carla F.; Quelemes, Patrick Veras; Oliveira, Mayara; Alves, A.; Pueta, Mariana; Gameiro, Paula; Tomás, Ana M.; Delerue-Matos, Cristina; Eaton, Peter; Camperi, Silvia A.; Basso, Néstor G.; Leite, Jose Roberto de Souza de Almeida, Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli. Gene, 605, 70-80, 2017|
|Abstract(s):||Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 M). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.|
|Access:||Restricted access (UMinho)|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|
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