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|Title:||A two-component pre-seeded dermal-epidermal scaffold|
|Author(s):||Monteiro, I. P.|
Timko, B. P.
Marques, A. P.
Reis, R. L.
Kohane, D. S.
|Citation:||Monteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014|
|Abstract(s):||We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|