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TitleA two-component pre-seeded dermal-epidermal scaffold
Author(s)Monteiro, I. P.
Gabriel, D.
Timko, B. P.
Hashimoto, M.
Karajanagi, S.
Tong, R.
Marques, A. P.
Reis, R. L.
Kohane, D. S.
KeywordsSkin defects
Hyaluronic acid
Amine–aldehyde bonding
Issue dateSep-2014
JournalActa Biomaterialia
CitationMonteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014
Abstract(s)We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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