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https://hdl.handle.net/1822/44424| Título: | Trypsin purification using magnetic particles of azocasein-iron composite |
| Autor(es): | Alves, Maria Helena Nascimento, Gabriela Ayres Cabrera, Mariana Paola Silvério, Sara C. Gonçalves, Clarisse Salomé Nobre Teixeira, J. A. Júnior, Luiz Bezerra de Carvalho |
| Palavras-chave: | Enzyme purification Proteases Fish viscera Azocasein iron oxides |
| Data: | Jul-2017 |
| Editora: | Elsevier |
| Revista: | Food Chemistry |
| Citação: | Alves, M.; Nascimento, Gabriela Ayres; Cabrera, Mariana Paola; Silvério, Sara C.; Nobre, Clarisse; Teixeira, J. A.; Júnior, Luiz Bezerra de Carvalho, Trypsin purification using magnetic particles of azocasein-iron composite. Food Chemistry, 226, 75-78, 2017 |
| Resumo(s): | This work presents an inexpensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagnetic particles of azocasein composite (mAzo). Crude extract was obtained from intestines of fish Nile tilapia (Oreochromis niloticus) homogenized in buffer (01 g tissue/ml). This extract was exposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field. Trypsin was leached off under high ionic strength (3 M NaCl). Preparation was achieved containing specific activity about 60 times higher than that of the crude extract. SDS-PAGE showed that the purified protein had molecular weight (24 kDa) in concordance with the literature for the Nile tilapia trypsin. The mAzo composite can be reused and applied to purify trypsin from other sources. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/44424 |
| DOI: | 10.1016/j.foodchem.2016.12.094 |
| ISSN: | 0308-8146 |
| Versão da editora: | http://www.elsevier.com/locate/issn/03088146 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| document_46550_1.pdf | 335,87 kB | Adobe PDF | Ver/Abrir |
